A canonical case is comprised of the following:
- A single, frozen, solid, primary, untreated tumor and single normal sample, with no other samples distributed. This is the vast majority of cases in TCGA.
- For liquid tumors, a single tumor sample and single normal sample, with no other samples distributed.
- For melanoma cases, a single, untreated, frozen, primary or metastatic tumor sample and a single normal sample.
- Analytes distributed for the following core characterizations:
- Whole exome DNA sequence analysis
- SNP 6.0 DNA microarray analysis
- DNA methylation analysis
- mRNA expression analysis by microarray or RNASeq
- miRNA expression analysis by miRNASeq
- DNA low-pass sequence analysis is neither necessary nor sufficient to count toward being canonical.
- The location of DNA isolation (BCR or TSS) for normal blood has no effect on being canonical.
- Cases comprised of only fixed-formalin paraffin embedded (FFPE) samples are not considered canonical for the purposes of TCGA.
- "Canonical" status is not necessarily permanent. If additional samples (e.g. a metastasis) are added to the case and analytes from them are shipped, the case would be promoted to "Canonical-Plus."
- 1 primary frozen untreated tumor sample AND 1 germline blood normal
- 1 primary frozen untreated tumor sample AND 1 untreated solid tissue-derived normal