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What is ISA-TAB?

Investigation-Study-Assay tab-delimited format (ISA-TAB) is a format based on the ISA-TAB specification Image Added that is used to capture and communicate the complex metadata required to interpret investigations (experiments) employing combinations of technologies. Metadata in ISA-TAB format facilitates standards-compliant collection, curation, management, and reuse of datasets in a wide variety of life science domains. ISA-TAB builds on the existing paradigm that is Microarray Gene Expression - Tabular format (MAGE-TAB)-a tab-delimited format to exchange microarray data.

Investigations, Studies, and Assays are the three entities around which the ISA-TAB framework is built. Investigation is a higher-order object, whose primary role is to group related Studies. Study is the central unit, containing information on the subject under study, its characteristics and any treatments applied. A Study has associated Assays. Assays are measurements performed either on the whole initial subject or on a sample taken from the subject, which produce qualitative or quantitative data. Assays can be characterized as the smallest complete unit of experimentation producing data associated with a subject.

The ISA-TAB specification relies on the ISA data model to order and classify the metadata. The ISA data model uses the following three types of text files to capture the metadata:

• The Investigation file contains all of the information needed to understand the project context, overall goals, and means. An Investigation is a high-level concept that groups related Studies.
• The Study file(s) includes information on the subject under study, its characteristics, and any treatments applied. A Study is the central unit and has associated Assays.
• The Assay file(s) include test steps and analytical measurements. An Assay is a test performed either on material taken from the subject or the whole initial subject, which produces qualitative and quantitative measurements (data).

Each of these files is a tab-delimited text file that researchers can create, view, and edit.

For more information about how the Investigation, Study, and Assay data are linked in the ISA-TAB format, refer to LinkedISA: Semantic Representation of ISA-Tab Experimental Metadata.

Introduction to the CSSI DCC Portal

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