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1. Create or import an investigation, as described in one of the following pages:
   • Creating an Investigation
   • Importing an Existing Investigation
2. Add or edit items, as described in one of the following pages:
   • Adding Items
   • Editing Items
3. In the Action panel, click Save ISA TAB Investigation. The system performs a validation check. 
   • If the investigation fails validation, a message appears requesting your confirmation. If you save the investigation with validation errors, you might not be able to import the resulting investigation file. To save anyway, click Save.
   • After the validation check, a dialog box appears for saving the investigation. Navigate to and select the folder in which you want to save the investigation. The recommended filename for the investigation is i_investigation.txt. Click Save. 

The system saves the following tab-delimited text files to the specified location:

• One i_investigation.txt file for the ISA Tab Investigation. 
• One file for each study, with a file name as specified in the Study File Name field.
• One file for each assay, with a file name as specified in the Study Assay File Name field.

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