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The Assay File will vary depending on the type of assay performed and protocol and technology type leveraged.  Provided below are examples  The following sections provide examples of Assay Files for common types of assays identified across nanotechnology resources. Assay File examples were created from assays and protocols performed by the Nanotechnology Characterization Laboratory (NCL) and identified in the NCL assay cascade.

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Hemolysis refers to the breaking open of red blood cells causing release of hemoglobin into the plasma.  The Hemolysis assay measures the amount of hemoglobin in whole blood (TBH-Total Blood Hemoglobin) and the amount of hemoglobin released into plasma (PFH-Plasma Free Hemoglobin) when blood is exposed to nanoparticles.  Hemoglobin is oxidized to methemoglobin and when reacting with cyanide results in Cyanmethemoblobin (CMH). The hemolysis assay is used to evaluate can quantify the acute in vitro hemolytic properties of nanoparticles.

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