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Comment: Revised based on reply from Sunita.
  1. Transfer your dataset from DME to the dme-sra-submit-aws S3 bucket, as described in Downloading to an AWS S3 Bucket via the GUI. (For credentials to this bucket, contact ncidatavault@mail.nih.gov.) AWS keeps this data in this bucket for two weeks. 

  2. In SRA (https://submit.ncbi.nlm.nih.gov/subs/sra) after login, select the option to Upload via Cloud using Amazon S3.

    SRA upload options.Image Modified

    The bucket policy is already set for NCBI to access the dataset. 

  3. Follow the instructions indicated on this page:

    https://docs.aws.amazon.com/AmazonS3/latest/userguide/add-bucket-policy.html

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  4. In the SRA Files tab, specify the name of this S3 bucket as the source of your files.  

    SRA submission page.

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