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  1. Transfer your dataset from DME to the dme-sra-submit-aws S3 bucket, as described in Downloading to an AWS S3 Bucket via the GUI. (For credentials to this bucket, contact ncidatavault@mail.nih.gov.) AWS keeps this data in this bucket for two weeks. 

  2. In SRA (https://submit.ncbi.nlm.nih.gov/subs/sra) after login, click New submission.

    SRA Submission Portal.Image Removed

    The  The bucket policy is already set for NCBI to access the dataset. 

  3. In the SRA Files tab, specify dme-sra-submit-aws as the name of this S3 bucket as the source of your files.  

    SRA submission page.

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