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Sample Name

Assay Name

Measurement Value[hydrodynamic diameter]

Statistic

Unit

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NCL-20-Sample1

size by DLS assay

5.2

z-average

nm

NCL-20-Sample2

size by DLS assay

8.6

z-average

nm

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Nano-TAB Extensions---In support of nano-TAB, the extensions and constraints in Table 6 TABLE 2 were applied to the ISA-TAB assay file and are further described in Section 5.6.  One important extension is that  nano-TAB allows the entry of summary data in the assay files through an optional field called Measurement Value, as described in TABLE 6.

The assay file is a horizontally oriented spreadsheet, where the field names are organized as column headers in the first row. TABLE 2325, TABLE 2426, and TABLE 2527, show an example of an assay file.

According to the ISA-TAB specification, the assay file has different types of fields called nodes, attributes of nodes, qualifiers of nodes’ attributes, and other valid fields.     The assay file supports the following types of information:

  •  Source or sample name;
  • Assay protocol reference and protocol parameter values;
  • Assay name, performer, and date the assay was performed;
  • Assay factor values including units and statistics, if applicable;
  • Assay measurement values including units and statistics, if applicable; and
  • Raw data files, derived data files or image files, if applicable.

Nodes

Source Name---The unique identification name of the source referred to from within the study file.  This column is used only if the source is same as the sample in an assay. Source names can be qualified only using Comment.

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