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Zeta Potential Assay
Particle surface characteristics and charge play an important role in the particle’s physical state, stability in different media, agglomeration tendencies, and interaction with biological systems. Zeta potential measurement provides an indirect measure of the net charge and as a tool to test batch-to-batch consistency.
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In Vitro Characterization Assays
Cytotoxicity Assay
Hepatocarcinoma Cytotoxicity MTT Assay
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Hemolysis refers to the breaking open of red blood cells causing release of hemoglobin into the plasma. The Hemolysis assay measures the amount of hemoglobin in whole blood (TBH-Total Blood Hemoglobin) and the amount of hemoglobin released into plasma (PFH-Plasma Free Hemoglobin) when blood is exposed to nanoparticles. Hemoglobin is oxidized to methemoglobin and when reacting with cyanide results in Cyanmethemoblobin (CMH). The hemolysis assay is used to evaluate the acute in vitro hemolytic properties of nanoparticles.
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