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Source Name---The unique identification name of the biological source from where the sample is derived. If the sample assayed is a biological specimen, its source is the starting biological material from which the sample was derived after the application of a protocol. The name of the source typically refers to the cell line or animal number for biological specimens. Source names can be qualified using the following column attributes: characteristic [ ], material type, material file, provider, and comment.

Material Source Name ReferenceREF---The reference to the material source name specified in the Material file.  The material source name should match the value recorded for Material Source Name in the corresponding ISA-TAB-Nano Material file. The material source name identifies the source from where the material sample is derived.  If the sample assayed is a nanoparticle sample or some chemical substance not derived from a biological material, then the corresponding material source name should refer to the starting sample that was modified by a protocol for the assay.  The material source name column should only be used for physical-chemical or other characterizations in which the material is the primary sample. For in vitro and in vivo characterizations in which the biological specimen is the primary sample, the material should be referenced as a material factor (e.g. Factor Value [material]).

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One or more attributes are used to provide more information about a study node. The different node attributes are described below:

Material typeType---An attribute for the source name or sample name (for example, biospecimen, nanoparticle sample, small molecule etc.). The term can be a free-text description or taken from an ontology or a controlled vocabulary. If it is the latter, then the following qualifiers are used: term accession number and term source REF.

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Date---The calendar day on which the protocol was carried out. The date format should be in YYYY-MM-DD. If there are other dates to be recorded (for example, the date when a sample was received from a vendor), one should create a comment [ ] column and specify the type of date within the square brackets (e.g., Comment [date received]).

Parameter value Value [<parameter term]---Value of a parameter, which is kept constant, when applying a protocol. The parameter term is written within brackets and must match the term used as value for the study protocol parameter name in the ISA-TAB-Nano investigation file.

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