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Screening was carried out in a 384-well plate format at a single point concentration of 10 µg/mL with ≥2 replicates per well, and results of the HTS on 326,656 NPNPD fractions is available for download. Furthermore, confirmation of single-point hits was done in eight-point dose-response at a concentration of 0.08 to 10 µg/mL with the results also available for download. Summary of the screening methodology, analysis of the results of the HTS, as well as a small proof-of-concept study that demonstrates the identification of new natural products with selective anti-bacterial and anti-fungal activities can be found in Martinez-Fructuoso et al.¹ For further information, please contact the Natural Products Branch at NCINatProdRep@mail.nih.gov.

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1. Martínez-Fructuoso, L., Arends, S. J. R., Freire, V. F., Evans, J. R., DeVries, S., Peyser, B. D., Akee, R. K., Thornburg, C. C., Kumar, R., Ensel, S., Morgan, G. M., McConachie, G. D., Veeder, N., Duncan, L. R., Grkovic, T., & O’Keefe, B. R. (2023). Screen for New Antimicrobial Natural Products from the NCI Program for Natural Product Discovery Prefractionated Extract Library. ACS Infectious Diseases. https://pubs.acs.org/doi/10.1021/acsinfecdis.3c00067

FILES FOR DOWNLOAD

GENERAL COMMENTS REGARDING THE DOWNLOADABLE FILES

• Downloads are zip-archived comma-separated values (csv) files, containing results from all screened natural product extracts and fractions.

FILE COLUMN HEADERS

10

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µg/mL SINGLE-DOSE INHIBITION DATA

1. Sample ID: Sample ID for crude and prefractionated extracts. Digit following the underscore represents polarity of the prefractionation, from 1 (most polar) to 7 (least polar), or 0 for unfractionated crude extract. Initial letter represents sample type: C = marine animal, M = fractionated marine animal, F = microorganism, H = fractionated microorganism, J = marine plant, K = fractionated marine plant, N = non-marine plant, L = fractionated non-marine plant.
2. Parent Extract: Crude extract parent identifier for each prefractionated extract. Initial letter represents sample source (as above).
3. NIH Plate: Plate ID from NCI NPNPD
4. Well: well identifier within each 384-well plate
5. JMI Plate: Plate number for JMI screening
6. C. albicans Replicate 1: percent inhibition values for first replicate of tests for C. albicans
7. C. albicans Replicate 2: percent inhibition values for second replicate of tests for C. albicans
8. C. albicans Replicate 3: percent inhibition values for third replicate of tests of some plates for C. albicans
9. C. albicans Replicate 4: percent inhibition values for fourth replicate of tests of some plates for C. albicans
10. E. coli (wild-type) Replicate 1: percent inhibition values for first replicate of tests for E. coli
11. E. coli (wild-type) Replicate 2: percent inhibition values for second replicate of tests for E. coli
12. E. coli (wild-type) Replicate 3: percent inhibition values for third replicate of tests of some plates for E. coli
13. E. coli (wild-type) Replicate 4: percent inhibition values for fourth replicate of tests of some plates for E. coli
14. E. coli (tolC) Replicate 1: percent inhibition values for first replicate of tests for E. coli tolC mutant
15. E. coli (tolC) Replicate 2: percent inhibition values for second replicate of tests for E. coli tolC mutant
16. E. coli (tolC) Replicate 3: percent inhibition values for third replicate of tests of some plates for E. coli tolC mutant
17. E. coli (tolC) Replicate 4: percent inhibition values for fourth replicate of tests of some plates for E. coli tolC mutant
18. S. aureus Replicate 1: percent inhibition values for first replicate of tests for S. aureus
19. S. aureus Replicate 2: percent inhibition values for second replicate of tests for S. aureus
20. S. aureus Replicate 3: percent inhibition values for third replicate of tests of some plates for S. aureus

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