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1. Transfer your dataset from DME to the dme-sra-submit-aws S3 bucket, as described in Downloading to an AWS S3 Bucket via the GUI. (For credentials to this bucket, contact ncidatavault@mail.nih.gov.) AWS keeps this data in this bucket for two weeks. 

2. In SRA (https://submit.ncbi.nlm.nih.gov/subs/sra) after login, select the option to Upload via Cloud using Amazon S3.

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   The click New submission. The bucket policy is already set for NCBI to access the dataset. 

3. Follow the instructions indicated on this page:

   https://docs.aws.amazon.com/AmazonS3/latest/userguide/add-bucket-policy.html

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4. In the SRA Files tab, specify dme-sra-submit-aws (the name of this the S3 bucket) as the source of your files.  

   

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