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Modified the ISA-TAB-Nano 1.0 1 version to address user comments. Modifications include:

Addition Removal of a MATERIAL section in the ISA-TAB Investigation File to reference the Material Linkage column from the Material File Name and Material Source Name.Removal of Material File in the Study File.
Removal of a separate field in the ISA-TAB Assay File to capture Statistics. Users should reference the Statistic in the Measurement Value parameter name in brackets (e.g. Measurement Value [Mean (diameter)]”).

Enforced the following business rule:
1. If the assay is a physico-chemical characterization, there should be no study factors of Study Factor Type nanoparticle sample
2. If the assay is a biological assay (in vitro, in vivo), the Study Factor Name and type must be of nanoparticle sample if the assay is applying a nanoparticle sample to a biological system.

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In the Study File, order Protocol REF and associated parameters immediately after Source Name and associated attributes and before Sample Name and Factor Values

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Currently, ISA-TAB does not support Study Grant Number and Study Funding Agency as standard fields in the Investigation Files. These fields can be added in a comments field.
Grouping of assays is subjective to the particular study. Recommend grouping of assays that use the same sample under one study.
For studies involving insertion of tumor cells into mice and the application of a nanoparticle as a treatment, the tumor cells should be parameter values and the nanoparticle should be factor values.

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