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The ISA-TAB-Nano Assay File leverages the ISA-TAB file format, which is a horizontal-based spreadsheet format with column headers and row values. An The following table provides an example subset of this format is provided in TABLE 1. Please note, concepts . Concepts specified within the bracket “[ ]” are variables depending on the type of assay.
TABLE 1: Example Subset of the Assay File Format
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Assay Name---The name of the assay performed. This name is used as an identifier within the assay file.
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Attributes of Assay Nodes
One or more attributes are can be used to provide more information about a assay node. The different assay node attributes are described below:
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Attributes of Processing Events for Assay Nodes
One or more attributes are can be used to describe a step in the assay of a sample.
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Attribute of Assay Name
One or more attributes are can be used to record the assay endpoints.
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Measurement Value [statistic(measurement name)]---The endpoint of the assay. Measurement value names are described in brackets or parenthesis if a statistics (e.g., such as mean, standard deviation) is applied to the measurement (Syntax: Measurement Value[statistic(<measurement name>)]). A statistic is only required for statistical measures.
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Unit---The standard of measurement used if the values for characteristic [ ], parameter value [ ], factor value [ ], or measurement value [ ] columns are quantitative and dimensional. If the term for unit is taken from an ontology/controlled vocabulary, then its term accession number and the term source REF should be defined in the ISA-TAB-Nano Assay File.
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Comment [ ]---Any comment that provides additional information, which is added only when no other appropriate field exists. Comment fields should be attached to the closest node or the field.
Files
The Assay File Examples, Template, and Glossary are provided below.following pages provide the assay file examples, template, and glossary:
