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(3) The investigation file provides descriptive information about studies including design descriptors, publications, factors, assays, protocols, and contacts.  This descriptive information lays the foundation for other nano-TAB files.  For example, TABLE 1 shows a subset of the Investigation File, which is the study factors section of the investigation file.  This section provides the names of factors (e.g., temperature, solvent medium) used in the study and their associated units of measurement (if the factors are quantitative).  The values of these factors (for example, PBS, 25 Celsius) are specified either in the study or the assay file.

File Format

---The nano-TAB investigation file leverages the ISA-TAB file format, which is a vertical-based spreadsheet format with row headers in the first column, as shown in TABLE 1. The fields are divided into sections. Therefore, the field values in the investigation file are entered in column order. For instance, in TABLE 1, Column A indicates the field names and Columns B and C contain the field values.

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