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                                                                                                                                                                                      Assay File Examples

Description

---The assay file provides references to assay results including measurements, raw data files, derived data files, image files, and other file types.  There can be multiple assay files per study.

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1.1.5 Example Assay File: Assay File for MTT Cytotoxicity--The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. TABLE 26 illustrates an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).

Assay File Examples