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_Sample Name_\---The unique identification name of the sample. The sample is obtained after the application of a protocol.  Sample names can be qualified using the following column attributes: _characteristic_ \[ \], _material type_, _material file_, and _comment_.

_Characteristic \[ \]_\---An attribute for _source name_ and _sample name_.  The term for each characteristic \[ \] attribute is written within brackets (e.g., Characteristic \[organism\], Characteristic \[cell type\], etc.).

_Date_\---The calendar day on which the protocol was carried out.  The date format should be in YYYY-MM-DD.  If there are other dates to be recorded (e.g., the date when a sample was received from a vendor), one should create a _comment \[ \]_ column and specify the type of date within the square brackets (e.g., Comment \[date received\]).

_Parameter value \[<parameter term\]_\---Value of a parameter, which is kept constant, when applying a protocol.&nbsp; The parameter term is written within brackets and must match the term used as value for the _study protocol parameter name_ in the nano-TAB investigation file.

_Unit_\---The standard of measurement used if the values for _characteristic_ \[ \], _parameter value \[ \]_ or _factor  value \[ \]_ columns are quantitative and dimensional. If the term for _unit_ is taken from an ontology/controlled vocabulary, then its _term accession number_ and the _term source REF_ should be defined in the nano-TAB investigation file.

_Factor Value \[<factor name>\]_\---The value of an independent variable manipulated by the experimentalist with the intention to affect the subject of study (i.e. stressor). Factor terms are given in brackets, and must be defined in the nano-TAB investigation file in the STUDY FACTORS section.

_Comment \[ \]_\---Any comment that provides additional information, which is added only when no other appropriate field exists.