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Sample Name

Assay Name

Measurement Value[hydrodynamic diameter]

Statistic

Unit

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NCL-20-Sample1

size by DLS assay

5.2

z-average

nm

NCL-20-Sample2

size by DLS assay

8.6

z-average

nm

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  •  Source or sample name;
  • Assay protocol reference and protocol parameter values;
  • Assay name, performer, and date the assay was performed;
  • Assay factor values including units and statistics, if applicable;
  • Assay measurement values including units and statistics, if applicable; and
  • Raw data files, derived data files or image files, if applicable.

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Nodes

Source Name---The unique identification name of the source referred to from within the study file.  This column is used only if the source is same as the sample in an assay. Source names can be qualified only using Comment.

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Wiki Markup
_Characteristic \[ \]_\---An attribute for _sample name_ if the same attribute is not given in the study file.

Assay name---The name of the assay performed. This name is used as an identifier within the assay file.

Attributes of Processing Events for Assay Nodes

processing events for assay nodes--- One or more attributes are used to describe a step in the assay of a sample.

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_Measurement value_ \[<measurement term>\]---The endpoint of the assay. &nbsp;This field is used to capture measurement outputs recorded in summary data.

Qualifiers for Assay Nodes’ Attributes

assay nodes’ attributes---Each node attribute may be qualified using the following concepts, if applicable.

Wiki Markup
_Unit_\---The standard of measurement used if the values for _characteristic_ \[ \], _parameter value \[ \]_ or _factor&nbsp; value \[ \]_ columns are quantitative and dimensional. If the term for _unit_ is taken from an ontology/controlled vocabulary, then its _term accession number_ and the _term source REF_ should be defined in the nano-TAB investigation file.

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_Term Accession Number_\---Identification number of a term selected from an ontology or a controlled vocabulary, if the term is entered as a value in _source name_, _material type,_ _characteristic \[ \]_, _parameter value \[ \]_, _unit_ or _factor value \[ \]_ columns.

Term Source REF---The name which identifies the source from where a term is selected and entered in nano-TAB study files.  This name should match one of the names entered in the term source name field in the nano-TAB investigation file.

Statistic---The type of statistical measure attributed to a numerical value (e.g., mean, standard deviation, z-average, etc.). This is a required field, if the parameter value, factor value, or measurement value is a statistical measure.

Other

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Assay Fields

Wiki Markup
_Factor Value\[<factor name>\]_\---The value of an independent variable manipulated by the experimentalist with the intention to affect the subject of study (i.e. stressor). Factor terms are given in brackets, and must be defined in the nano-TAB investigation file in the STUDY FACTORS section.&nbsp; Factor Value\[\] in assay file should reference technical variations (such as software, instrument or protocol variations).

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