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DLS is a type of spectroscopy that uses a laser beam to irradiate a sample containing particles in suspension resulting in light scattering. Rapid fluctuations in scattering intensity around a mean value at a certain angle occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusion coefficient, for a given temperature and viscosity, that can be used to calculate particle size. TABLE 25, TABLE 26, and TABLE 27 illustrate an example assay file for size by DLS.
TABLE 25 22 Assay File Example for Size by DLS Assay (Columns A-F)
TABLE 26 23 Assay File Example for Size by DLS Assay (Columns G-L)
TABLE 27 24: Assay File Example for Size by DLS Assay (Columns M-R)
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The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. TABLE 28 illustrates an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).
TABLE 2826: Assay File Example for MTT Cytotoxicity Assay in Porcine Proximal Tubule Cells.
