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File Format
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The nanoISA-TAB-Nano assay file leverages the ISA-TAB file format, which is a horizontal-based spreadsheet format with column headers and row values. An example subset of this format is provided in TABLE 21. Please note, concepts specified within the bracket “\[ \]” are variables depending on the type of assay. |
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A | B | C | D | E | ||
<ac:structured-macro ac:name="unmigrated-wiki-markup" ac:schema-version="1" ac:macro-id="7ae94770cb8ea978-6c3d57e8-41e44e18-9c23be51-8473bce78ff83e1fb4c86fcb"><ac:plain-text-body><![CDATA[ | Sample Name | Assay Name | Measurement Value[hydrodynamic diameter] | Statistic | Unit | ]]></ac:plain-text-body></ac:structured-macro> |
NCL-20-Sample1 | size by DLS assay | 5.2 | z-average | nm | ||
NCL-20-Sample2 | size by DLS assay | 8.6 | z-average | nm |
Fields
NanoISA-TAB-Nano Extensions---In support of nanoISA-TAB-Nano, the extensions and constraints in TABLE 2 were applied to the ISA-TAB assay file. One important extension is that nanoISA-TAB-Nano allows the entry of summary data in the assay files through an optional field called Measurement Value.
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_Unit_\---The standard of measurement used if the values for _characteristic_ \[ \], _parameter value \[ \]_ or _factor value \[ \]_ columns are quantitative and dimensional. If the term for _unit_ is taken from an ontology/controlled vocabulary, then its _term accession number_ and the _term source REF_ should be defined in the nanoISA-TAB-Nano investigation file. |
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_Term Accession Number_\---Identification number of a term selected from an ontology or a controlled vocabulary, if the term is entered as a value in _source name_, _material type,_ _characteristic \[ \]_, _parameter value \[ \]_, _unit_ or _factor value \[ \]_ columns. |
Term Source REF---The name which identifies the source from where a term is selected and entered in nanoISA-TAB-Nano study files. This name should match one of the names entered in the term source name field in the nanoISA-TAB-Nano investigation file.
Statistic---The type of statistical measure attributed to a numerical value (e.g., mean, standard deviation, z-average, etc.). This is a required field, if the parameter value, factor value, or measurement value is a statistical measure.
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_Factor Value\[<factor name>\]_\---The value of an independent variable manipulated by the experimentalist with the intention to affect the subject of study (i.e. stressor). Factor terms are given in brackets, and must be defined in the nanoISA-TAB-Nano investigation file in the STUDY FACTORS section. Factor Value\[\] in assay file should reference technical variations (such as software, instrument or protocol variations). |
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