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The assay file will vary depending on the type of assay performed and protocol and technology type leveraged.  Provided below are examples of assay files for two common types of assays identified across nanotechnology resources.

Example Assay File: Size by Dynamic Light Scattering (DLS)

DLS is a type of spectroscopy that uses a laser beam to irradiate a sample containing particles in suspension resulting in light scattering. Rapid fluctuations in scattering intensity around a mean value at a certain angle occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusion diffusio coefficient, for a given temperature and viscosity, that can be used to calculate particle size.   TABLE ABLE 25, TABLE 26, and TABLE 27 illustrate an example assay file for size by DLS.

TABLE 22 Assay File Example for Size by DLS Assay (Columns A-F)

TABLE 23 Assay File Example for Size by DLS Assay (Columns G-L)

TABLE 24: Assay File Example for Size by DLS Assay (Columns M-R)

Example Assay File: Assay File for MTT Cytotoxicity

The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. TABLE 28 illustrates an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).

TABLE 26: Assay File Example for MTT Cytotoxicity Assay in Porcine Proximal Tubule Cells.

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