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The assay file will vary depending on the type of assay performed and protocol and technology type leveraged. Provided below are examples of assay files for two common types of assays identified across nanotechnology resources.
Example Assay File: Size by Dynamic Light Scattering (DLS)
DLS is a type of spectroscopy that uses a laser beam to irradiate a sample containing particles in suspension resulting in light scattering. Rapid fluctuations in scattering intensity around a mean value at a certain angle occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusio coefficient, for a given temperature and viscosity, that can be used to calculate particle size. ABLE 25, TABLE 26, and TABLE 27 illustrate an example assay file for size by DLS.
TABLE 22 Assay File Example for Size by DLS Assay (Columns A-F)
TABLE 23 Assay File Example for Size by DLS Assay (Columns G-L)
TABLE 24: Assay File Example for Size by DLS Assay (Columns M-R)
Example Assay File: Assay File for MTT Cytotoxicity
The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
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bromide)
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to
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a
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product
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that
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has
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a
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purple
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color.
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This
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assay
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can
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measure
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the
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cytotoxicity
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of
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a
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chemical
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or
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drug
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by
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determining
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the
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affect
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of
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treatment
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on
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cell
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viability.
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TABLE
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28
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illustrates
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an
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example
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for
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a
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MTT
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cytoxicity
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assay
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(MTT)
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performed
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on
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three
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nanoparticle
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samples,
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where
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9
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dilutions
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of
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each
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nanoparticle
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sample
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are
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exposed
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to
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porcine
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proximal
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tubule
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cells
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for
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three
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different
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times
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of
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exposure
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(6h,
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2h,
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and
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48
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h).
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TABLE
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26:
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Assay
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File
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Example
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for
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MTT
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Cytotoxicity
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Assay
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in
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Porcine
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Proximal
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Tubule
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Cells.
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