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The assay file Assay File will vary depending on the type of assay performed and protocol and technology type leveraged.  Provided below are examples of assay files Assay Files for two common types of assays identified across nanotechnology resources.

Example Assay File: Size

Size by Dynamic Light Scattering (DLS)

Description: Dynamic Light Scattering (DLS) is a type of spectroscopy that uses a laser beam to irradiate a sample containing particles in suspension resulting in light scattering. Rapid fluctuations in scattering intensity around a mean value at a certain angle occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusio diffusion coefficient, for a given temperature and viscosity, that can be used to calculate particle size. ABLE 25, TABLE 26, and TABLE 27 illustrate an example assay file for size by DLS.

TABLE 22 Assay File Example for Size by DLS Assay (Columns A-F)

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TABLE 23 Assay File Example for Size by DLS Assay (Columns G-L)

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TABLE 24: Assay File Example for Size by DLS Assay (Columns M-R)

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Example Assay File: Assay File for MTT Cytotoxicity

Example Assay File(s):

• Size by DLS

Assay File Metadata:

• Factors: Temperature, Solvent Medium
• Measured Values: Hydrodynamic Diameter, Peak Size, PDI

Supporting Information: 

• Protocol: Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering
• Citation:  Characterization of Nanoparticles for Therapeutics

Example Assay File: Cytotoxicity

Hepatocarcinoma Cytotoxicity MTT Assay

Description: The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. TABLE 28 illustrates an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).

TABLE 26: Assay File Example for MTT Cytotoxicity Assay in Porcine Proximal Tubule Cells.

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Example Assay File(s):

• Hep G2 Hepatocarcinoma Cytotoxicity Assay

Assay File Metadata:

• Factors: Time
• Measured Values: Cell Viability

Supporting Information:

• Protocol: Hep G2 Hepatocarcinoma Cytotoxicity Assay Protocol
• Citation: Characterization of Nanoparticles for Therapeutics