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The Assay File will vary depending on the type of assay performed and protocol and technology type leveraged. Provided below are examples of Assay Files for common types of assays identified across nanotechnology resources.

Physico-Chemical Characterization Assays

Size Assay

Size by Dynamic Light Scattering (DLS) Assay

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Assay File	Assay Factors	Assay Measured Values	Supporting Information
Size by DLS	Temperature Solvent Medium	Hydrodynamic Diameter Peak Size Polydispersity Index (PDI)	Measuring the Size of Nanoparticles in Aqueous Solution using DLS Characterization of Nanoparticles for Therapeutics

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Physico-Chemical Characterization Assays

Size Assay

Size by Dynamic Light Scattering (DLS) Assay

In Vitro Characterization Assays

Cytotoxicity Assay

Hepatocarcinoma Cytotoxicity MTT Assay