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The ISA-TAB-Nano assay file leverages the ISA-TAB file format, which is a horizontal-based spreadsheet format with column headers and row values. An example subset of this format is provided in TABLE 211. Please note, concepts specified within the bracket “[ ]” are variables depending on the type of assay.

TABLE 211: Example Subset of the Assay File Format

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The assay file is a horizontally oriented spreadsheet, where the field names are organized as column headers in the first row. TABLE 22 - TABLE 25, and TABLE 26 show two examples of an assay file.

According to the ISA-TAB specification, the assay file has different types of fields called nodes, attributes of nodes, qualifiers of nodes’ attributes, and other valid fields. The assay file supports the following types of information:

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