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The Disposition and Pharmacokinetics assay is assay is performed to detect the nanoparticles (or their components) in tissues taken at various time pointsdetermine the fate of a nanoparticle formulation in an organism, which can include its absorption, tissue distribution, metabolism or excretion (ADME). This is accomplished by detecting the whole formulation or its components at various body locations and time points after dosing. A variety of methods can be used to detect and quantify nanoparticles in tissuenanoparticle formulations and their components in vivo.