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In Vitro Characterization Assays
Cytotoxicity Assay
Hepatocarcinoma
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Cytotoxicity Assay (MTT and LDH)
The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. The Assay File represents an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).
Assay File | Assay Factors | Assay Measured Values | Supporting Information |
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Hep G2 Hepatocarcinoma Cytotoxicity Assay | |||
Hep G2 Hepatocarcinoma Cytotoxicity Assay |
Blood Contact Assays
Hemolysis Assay
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