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The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. The Assay File represents an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).

LDH is a cytoplasmic enzyme that is released into the cytoplasm upon cell lysis. The LDH assay is a measure of membrane integrity. The basis of the LDH assay: (1) LDH oxidizes lactate to pyruvate, (2) Pyruvate reacts with the tetrazolium salt INT to form formazan, and (3) the water-soluble formazan dye is detected spectrophotometrically.

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