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Assay FileAssay FactorsAssay Measured ValuesSupporting Information
Hep G2 Hepatocarcinoma Cytotoxicity Assay
Hep G2 Hepatocarcinoma Cytotoxicity Assay

Caspase Apoptosis

Caspase Apoptosis assays monitor apoptosis by measuring the degree of the caspasecaspace (family of cysteine proteases) activity, an indicator of apoptosis.   Caspase Apoptosis apoptosis assays measure the cleavage of the DEVD-AFC (Amino Tri-fluoromethyl   Coumarin ) substrate to free AFC.   Free AFC emits yellow-green fluorescence.

 

Assay FileAssay FactorsAssay Measured ValuesSupporting Information
Caspase Apoptosis
  • % Control Caspase Activity

Blood Contact Assays

Hemolysis Assay

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Assay FileAssay FactorsAssay Measured ValuesSupporting Information
Pharmacokinetics
  • Clearance at Time Zero (C0)
  • Area Under Curve (AUC)
  • Clearance (CL)
  • Half Life (T 1/2)
  • Volume of Distribution (V app)
Tissue Distribution
  • Tissue Distribution 

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  1. ISO 10993-5 Biological evaluation of medical devices: Part 5 Tests for in vitro  cytotoxicity.
  2. F1903 – 98 Standard Practice for Testing for Biological Responses to Particles in vitro  .
  3. Alley, et al. (1988) Cancer Res.  48:589-601.
  4. Decker, T. & Lohmann-Matthes, M.L. (1988) J. Immunol Methods  15:61-69.
  5. Korzeniewski, C. & Callewaert, D.M. (1983) J. Immunol Methods  64:313-320.