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Caspase Apoptosis assays monitor apoptosis by measuring the degree of the caspace (family of cysteine proteases) activity, an indicator of apoptosis. Caspase apoptosis assays measure the cleavage of the DEVD-AFC (Amino Tri-fluoromethyl Coumarin substrate to free AFC. Free AFC emits yellow-green fluorescence (1,2,6).

Assay FileAssay FactorsAssay Measured ValuesSupporting Information
Caspase Apoptosis
  • % Control Caspase Activity

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  1. ISO 10993-5 Biological evaluation of medical devices: Part 5 Tests for in vitro  cytotoxicity.
  2. F1903 – 98 Standard Practice for Testing for Biological Responses to Particles in vitro  .
  3. Alley, et al. (1988) Cancer Res.  48:589-601.
  4. Decker, T. & Lohmann-Matthes, M.L. (1988) J. Immunol Methods  15:61-69.
  5. Korzeniewski, C. & Callewaert, D.M. (1983) J. Immunol Methods  64:313-320.

 

 

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  1. Wang et al. (2005) Cell Bio. Int. 29: 489-496.