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RPKM is a method of quantifying gene expression from RNA sequencing data by normalizing for total read length and the number of sequencing reads.

Reference:

Mortazavi, A., Williams, B. A., McCue, K., Schaeffer, L., and Wold, B. (2008).
Mapping and quantifying mammalian transcriptomes by rna-seq.
Nat Methods, 5(7):621-628.

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