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Source NameThe unique identification name of the source from where the sample is derived. If the sample assayed is a biological specimen, its source is the starting biological material from which the sample was derived after the application of a protocol. The name of the source typically refers to the cell line or animal number for biological specimens. If the sample assayed is a nanoparticle sample or some chemical substance not derived from a biological material, then the corresponding source name should refer to the starting sample that was modified by a protocol for the assay. Source names can be qualified using the following column attributes: characteristics [ ], material type, provider, and comment.
Material TypeAn attribute for the source name or sample name (for example, biospecimen, nanoparticle sample, small molecule etc.). The term can be a free-text description or taken from an ontology or a controlled vocabulary. If it is the latter, then the following qualifiers are used: term accession number and term source REF.
Characteristics[ ]An attribute for source name and sample name. The term for each characteristics [ ] attribute is written within brackets (for example, Characteristics [organism], Characteristics [cell type], etc.).
ProviderAn attribute for source name, which refers to the name of the person or the vendor providing the source sample.
Protocol REFThe name of the protocol used to prepare the sample. This name should match a value for the field study protocol name in the investigation file (within the study protocols section).
Parameter Value[parameter name]Value of a parameter, which is kept constant, when applying a protocol. The parameter name is written within brackets and must match the term used as value for the study protocol parameter name in the ISA-TAB-Nano investigation file.
UnitThe standard of measurement used if the values for characteristic [ ], parameter value [ ] or factor value [ ] columns are quantitative and dimensional. If the term for unit is taken from an ontology/controlled vocabulary, then its term accession number and the term source REF should be defined in the ISA-TAB-Nano Study File.
Term Accession NumberIdentification number of a term selected from an ontology or a controlled vocabulary, if the term is entered as a value in source name, material type, characteristics [ ], parameter value [ ], unit or factor value [ ] columns.
Term Source RefThe name which identifies the source from where a term is selected and entered in ISA-TAB-Nano study files. This name should match one of the names entered in the term source name field in the ISA-TAB-Nano Investigation file.
PerformerThe name of the person who carried out the protocol.
DateThe calendar day on which the protocol was carried out. The date format should be in YYYY-MM-DD. If there are other dates to be recorded (for example, the date when a sample was received from a vendor), one should create a comment [ ] column and specify the type of date within the square brackets (e.g., Comment [date received]).
Sample NameThe unique identification name of the sample. The sample is obtained after the application of a protocol Sample names can be qualified using the following column attributes: characteristics [ ], material type, and comment.
Factor Value[factor name]The value of an independent variable manipulated by the experimentalist with the intention to affect the subject of study (that is, stressor). Factor names are given in brackets, and must be defined in the ISA-TAB-Nano investigation file in the STUDY FACTORS section.
CommentAny comment that provides additional information, which is added only when no other appropriate field exists.