If you have read-only permissions, see Access the Sample Characterization Summary. If you are a curator with appropriate security permissions, you can create, update, and delete characterizations. |
This chapter describes how to review or ascribe characterizations to samples save in caNanoLab. Topics in this chapter include:
Sample characterization describe distinctive characteristics or essential features of the sample determined through analytical methods and records information associated with sample synthesis and properties. Samples can be characterized in caNanoLab by physical or chemical characteristics or by data derived under in vitro and in vivo conditions.
With the appropriate security permissions, you can
If the buttons do not display, you have read-only access to the item. |
With read-only access, you can review a summary of characterization information and annotations added to the sample.
To access characterization functions in the Navigation Tree
The Navigation Tree appears on the left sidebar and comprises functions which you can use to add annotations to the sample.
Click Characterization.
The All tab displays characterizations already added to the sample by category. Additional tabs show annotations added to the sample for each subcategory.
Once you access Characterizations in the Navigation Tree, you can add different types of information to the sample.
If you have read-only access, you can View a Characterizations Summary.
To add a physico-chemical characterization
Click All or the Physico-Chemical Characterizations tab and click Add next to Physico-chemical Characterization. Both tabs display five sections, but All provides customizations based what you select in the Characterization Type* field.
Follow these steps to fill in the characterization. Links are provided for additional details.
Section | What to Do |
---|---|
Physico-Chemical Characterization | Fill in the general information about the characterization. |
[Characterization] Properties | Displays only for Physical State, Shape and Solubility characterizations. Define properties for the characterization, if applicable. |
Design and Methods | Complete the fields describing techniques and instruments used to derive the data. |
Finding | Add data findings and supporting documentation relating to the sample. |
Analysis and Conclusions | Enter any relevant analyses and conclusions reached by the data. |
Copy to other Samples... | Select samples in the list to which you want this physico-chemical data transferrred. This option copies files and data to one or more selected samples "owned" by the same point of contact. |
When you finish, click Submit to save the data to the sample.
Fill in the following characterization information.
Field | Description |
---|---|
Characterization Type* | The Characterization Type is already selected as Physico-Chemical Characterization. |
Characterization Name* | You must select the name of the Characterization you want to add or select an "other" option to create a new or select from an existing, non-standard Assay Type.
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Assay Type | The field populates automatically with your selection in Characterization Name.
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Protocol Name – Version | If this field is available, select from the drop-down list the protocol from which the data is derived. A hyperlink of the protocol text file may appear. Click the hyperlink to open or save the file. |
Characterization Source | Select from the drop-down list or enter the source from which the characterization data is derived, such as a vendor or a laboratory (NCL). |
Characterization Date | Select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy. |
Continue to define Physico-Chemical Properties.
The [Characterization] Properties section opens only for the characterizations listed in the table below. Fill in the following Properties information as needed.
Characterization | Property Fields | Field Options |
---|---|---|
Physical State | Type | Select the appropriate type from among the following options: |
Shape Properties | Type (required) | Select from the drop-down list the appropriate shape type: |
Shape Properties | Aspect Ratio | Enter the shape aspect ratio. |
Shape Properties | Minimum,/Maximum Dimensions | Enter the minimum and maximum dimensions of the sample, as well as the units of measurement. |
Solubility Properties | Solvent | Select from the options or enter the name.
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Solubility Properties | Critical Concentration | Enter appropriate values for the critical concentration, then select the appropriate units for those values. |
Solubility Properties | Is Soluble? | Select Yes or No as to whether the solvent is soluble. |
Surface Properties | Is Hydrophobic? | Indicate with Yes or No whether the surface is hydrophobic. |
Continue to define Physico-Chemical Design and Methods.
Fill in the following Design and Methods information as needed.
Design and Methods Field | Description | |
---|---|---|
Design and Methods Description | Enter a description for the characterization design and methods.
| |
Technique and Instrument | ||
Technique* | ||
Abbreviation | ||
Description | Enter a description of the technical and instrument. | |
Instrument |
Once you enter the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.
Continue to define Physico-Chemical Findings.
In the Finding section, you can add one or more publications relevant to the sample, as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.
Click Add to expand the section, then add data and conditions and add a file.
In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information, such as laboratory conditions like pH or temperature that are part of your findings.
To define the data and condition matrix
Column Type "Datum" is selected with this characterization | Autopopulated Column Name Option[s] |
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Molecular Weight | |
Purity | |
Relaxivity | |
Size | |
Surface |
After adding data and conditions to the sample, continue to add a Physico-Chemical Characterization.
After adding one or more files, add data and conditions or if you have no derived data to add, add a Physico-Chemical Characterization.
In vitro characterization allows you to add characterizations for the nanomaterial component of the sample derived from analytical techniques performed under in vitro conditions.
To add an in vitro characterization
Click All or the In Vitro Characterizations tab and click Add next to In Vitro Characterization. Both tabs display five sections, but the All tab provide customizations based what you select in the Characterization* field.
Follow these steps to fill in the characterization. Links are provided for additional details.
Field/Section | What to Do |
---|---|
In Vitro Characterization | Fill in the general information about the characterization. |
[Characterization] Properties] | Displays only for Cytotoxicity, Enzyme Induction and Transfection in vitro characterizations. Define properties for the characterization, if applicable. |
Design and Methods | Complete the fields describing techniques and instruments used to derive the data. |
Finding | Add data findings and supporting documentation relating to the sample. |
Analysis and Conclusions | Enter any relevant analyses and conclusions reached by the data. |
Copy to Other Samples... | Select samples in the list to which you want this physico-chemical data transferred. This option copies files and data to one or more selected samples "owned" by the same point of contact. |
When you finish, click Submit to save the data to the sample.
Fill in the following characterization information.
Field | Description |
---|---|
Characterization Type* | The Characterization Type is already selected as In Vitro Characterization. |
Characterization Name* | You must select the name of the Characterization you want to add or select an "other" option to create a new or select from an existing, non-standard Assay Type.
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Assay Type | The field populates automatically with options displayed for in vitro characterization selections: Blood Contact, Cytotoxicity, Immune Cell Function, Oxidative Stress, Sterility and Targeting.
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Protocol Name – Version | If available, select the protocol from which the data is derived.
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Characterization Source | Select or enter the source from which the characterization data is derived, such as a vendor or a laboratory (NCL). |
Characterization Date | Select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy. |
Continue to In Vitro Properties.
Specify one of the following in vitro Assay Type options or select [Other] to open a window where you can add a new assay type.
Characterization Selection | Assay Type Corresponding to Characterization |
---|---|
Blood Contact | |
Cytotoxicity | |
Immune Cell Function | |
Oxidative stress | |
Sterility | |
Targeting | |
Transfection | Click [Other] to add an assay type. |
Continue to define in vitro characterizations.
The [Characterization] Properties section opens only when you select the following characterizations in the Summary section. To define properties for each unique characterization, enter information for the following fields.
For Cytotoxicity, enter the appropriate Cell Line.
For Targeting, enter the appropriate Cell Line.
Continue to define In Vitro Design and Methods.
Fill in the following Design and Methods information as needed.
Design and Methods Field | Description | |
---|---|---|
Design and Methods Description | Enter a description for the characterization design and methods.
| |
Technique and Instrument | ||
Technique* | ||
Abbreviation | ||
Description | Enter a description of the technique and instrument. | |
Instrument |
Once you enter the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.
Continue to define In Vitro Findings.
In the Finding sections, you can add one or more publications relevant to the sample, as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.
Click Add to expand the section, then add data and conditions and add a file.
In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information, such as laboratory conditions that are part of your findings.
To define the matrix for the data
Column Type "Datum" is selected with this characterization | Autopopulated Column Name Option[s] |
---|---|
Enzyme Induction |
After adding data and conditions to the sample, add an In Vitro Characterization.
In vivo characterization allows you to add characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vivo conditions.
To add an in vivo characterization
Click the All or In Vivo Characterizations tab and click Add next to In Vivo Characterizations. Both tabs display five sections, but the All tab provide customizations based what you select in the Characterization* field.
Follow these steps to fill in the characterization. Links are provided for additional details.
Field/Section | What to Do |
---|---|
In Vivo Characterization | Fill in the general information about the characterization. |
[Characterization] Properties | Displays for in vivo characterizations. |
Design and Methods | Complete the fields describing techniques and instruments used to derive the data. |
Findings | Add data findings and supporting documentation relating to the sample. |
Analysis and Conclusions | Enter any relevant analyses and conclusions reached by the data. |
Copy to Other Samples... | Select samples in the list to which you want this physico-chemical data transferred. This option copies files and data to one or more selected samples "owned" by the same point of contact. |
When you finish, click Submit to save the data to the sample.
Fill in the following characterization information.
Field | Description | |
---|---|---|
Characterization Type* | The Characterization Type is already selected as In Vivo Characterization. | |
Characterization Name* | You must select the name of the Characterization you want to add or select an "other" option to create a new or select from an existing, non-standard Assay Type.
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Assay Type | Leave this blank or select a type.
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Protocol Name – Version | Select from the drop-down list the protocol from which the data is derived. A hyperlink of the protocol text file may appear. Click the hyperlink to open or save the file. | |
Characterization Source | Select from the drop-down list or enter the source from which the characterization data is derived, such as a vendor or a laboratory (NCL). | |
Characterization Date | Select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy. |
Continue to define In Vivo Design and Methods.
Fill in the following Design and Methods information as needed.
Design and Methods Field | Description | |
---|---|---|
Design and Methods Description | Enter a description for the characterization design and methods.
| |
Technique and Instrument | ||
Technique* | ||
Abbreviation | ||
Description | Enter a description of the technique and instrument. | |
Instrument |
Once you specify the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.
Continue to define In Vivo Findings.
In the Finding sections, you can add one or more publications relevant to the sample as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.
Complete the instructions as described in Adding Data and Conditions and Adding a File.
In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information that are part of your findings.
To define the matrix for the data
For an in vivo characterization, click [Other] to name the column yourself.
After adding one or more files, continue by Adding Data and Conditions or if you have no derived data to add, return to the steps described in Adding an In Vivo Characterization.
From a characterization summary page, and with curator privileges, you can perform these tasks:
To print a characterization
Click Print at the top right of the page.
If the button described in this section does not display, you have read-only access to the data. |
To export a characterization
Click Export at the top right of the page.
If the button described in this section does not display, you have read-only access to the data. |
To edit a characterization
Click Edit at the right of the characterization section you want to change. This opens the corresponding Characterization page where you can edit the file by following the same directions as described for creating characterizations.
If the button described in this section does not display, you have read-only access to the data. |
The characterization files and/or derived data for this sample can be copied to other samples from the same primary point of contact.
To copy characterizations, open the sample characterization form that displays the characterization
Click Submit to copy the characterization.
If the button described in this section does not display, you have read-only access to the data. |
To delete a characterization for a selected sample
This only deletes the characterization(s) for the selected sample. If the characterization(s) was copied to other samples, the characterization is not removed from those samples. |
Click Delete.
If the button described in this section does not display, you have read-only access to the data. |
Deleted characterizations are placed in the sample archive for history purposes.