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Question: What are the MAGE-TAB Files?
Topic: caArray Usage
Release: caArray 2.X
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Answer
MAGE-TAB (MicroArray Gene Expression Tabular) format files refer to simple tab-delimited, spreadsheet-based files, which can be used for annotating and communicating microarray data in a MIAME compliant fashion. MAGE-TAB specification is based on the Microarray and Gene Expression Object Model (MAGE-OM). MAGE-TAB specification and related publications provide more details on the format.
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Note that a MAGE-TAB format Array Design File (ADF) is not mandatory in caArray, since array design files for the common arrays are usually available from their respective array vendors. If an array design file (which may not be in MAGE-TAB format) is available from its array provider, you should choose it over an ADF. Third party array design files are uploaded using the Manage Array Design interface under caArray's Curation tab. An ADF file, on the other hand, can be uploaded together with the rest of MAGE-TAB files. An ADF file is not be validated or parsed by caArray. It will be imported directly into caArray according to the table that follows.
MAGE-TAB Formatted Files
Abbreviation | File Type | Description | caArray compatible? | Processed by caArray? |
|---|---|---|---|---|
IDF | Investigation Design File | Provides an overview of the experiment, including the experimental variables (factors) used, protocols, quality control strategy, publication information and contact details | Yes | Yes: Parsed, Validated before import |
SDRF | Sample Data Relationship File | Describes relationships between samples, arrays, data files, protocols, factor values etc. It is a table in which each hybridization channel is represented by a row, and columns represent the steps of the experiment. The ordering of these columns is important, and reads left-to-right in chronological order. | Yes | Yes: Parsed, Validated before import |
ADF | Array Design File | Provides the array-level annotation for the experiment. It relates the row-level identifiers in the data files to biological sequence annotation | Yes | No. Directly Import |
TXT or other | Data Matrix | Contains processed array data files in tab-delimited text format. Rows may represent genes/ exons/ genomic locations. Columns represent samples or experimental conditions. | Yes | No. Directly Import |
What are MAGE-TAB Files?
The term of "MAGE-TAB Files" (refer to caArray 002 - How do I upload MicroArray Gene Expression Data into caArray?, step 2 for an example), has been used to refer not only MAGE-TAB formatted files as summarized in the table, but also files that are supported by MAGE-TAB parser mentioned in the last section. To be more specific, MAGE-TAB files also include the third party's raw array data files, derived data files and array design files from array providers, as shown in the illustration.
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Descriptive files can contain information about investigation data or array design. Investigation data files conform to one of two specifications: Investigation Description File (IDF) or Sample and Data Relationship File (SDRF). Array design files conform to one of two specifications: MAGE-TAB formatted design files with a .ADF file extension, or non-MAGE-TAB formatted design files from third-party vendors that can still be parsed by caArray.
Building MAGE-TAB Formatted files
Because of the varied nature of every caArray experiment and its corresponding data, it is impossible to outline the exact steps for creating a caArray-compatible MAGE-TAB file. There are strict guidelines, however, for characteristics of MAGE-TAB files that meet the criteria. Refer to Appendix A - MAGE-TAB in caArray in the caArray User's Guide for specific details regarding caArray-compatible MAGE-TAB files. To provide context, see the MAGE-TAB Specification document.
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For more information about when to use MAGE-TAB annotation files in caArray, refer to caArray 008 - How do I use the Annotation Tab versus MAGE-TAB Annotation Files in caArray?. For more information on how to upload MAGE-TAB files, refer to Importing MAGE_TAB Files in the caArray User's Guide.
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