 |
Page History
...
This chapter describes how to review or ascribe characterizations to samples save in caNanoLab. Topics in this chapter include:
| Table of Contents | ||||
|---|---|---|---|---|
|
...
Sample characterization describe distinctive characteristics or essential features of the sample determined through analytical methods and records information associated with sample synthesis and properties. Samples can be characterized in caNanoLab by physical or chemical characteristics or by data derived under in vitro and in vivo conditions.
| Info | ||||
|---|---|---|---|---|
| ||||
With the appropriate security permissions, you can
If the buttons do not display, you have read-only access to the item. |
...
With read-only access, you can review a summary of characterization information and annotations added to the sample.
To access characterization functions in the Navigation Tree
...
The Navigation Tree appears on the left sidebar and comprises functions which you can use to add annotations to the sample.
...
Click Characterization.
The All tab displays characterizations already added to the sample by category. Additional tabs show annotations added to the sample for each subcategory.
...
Once you access Characterizations in the Navigation Tree, you can add different types of information to the sample.
- Adding a Physico-chemical Characterization
- Adding an In Vitro Characterization
- Adding an In Vivo Characterization
- Adding an Ex Vivo Characterization
- Adding an Other Ex Vivo
If you have read-only access, you can View a Characterizations Summary.
...
Exporting Sample Information to JSON or XML
...
...
To add a physico-chemical characterization
...
Click the All tab or the Physico-Chemical Characterizations tab and click Add next to Physico-chemical Characterization.
...
Both tabs provide customizations based on your Characterization Type* selection.
...
If you select one of the following characterization types, you need to fill in additional properties.
- Physical State
- Shape
- Solubility
- Surface
...
Add data findings and supporting documentation relating to the sample. Import as many .csv files as you wish or add derived data manually.
Click Add to expand the section, and add data findings and add supporting documents to the characterization.
...
Copy to Other Samples with the Same Primary Organization
...
When you are finished, click Submit to save the data to the sample.
...
If you selected one of the Characterization Names listed in the following table, the Properties section opens, and you must fill in additional information.
...
Physical State
...
Select the Type of physical state.
...
Shape
...
Select the Type of shape.
- Enter the shape Aspect Ratio.
- Enter the Minimum and Maximum Dimensions of the sample, and the units of measurement.
...
Solubility
...
- Select from the options or enter the name of the Solvent.
- Enter appropriate values for the Critical Concentration, then select the appropriate units for those values.
- Specify Yes or No to indicate whether the solvent Is Soluble?.
...
Surface
...
Specify Yes or No to indicate whether the surface Is Hydrophobic?.
...
In vitro characterization allows you to add characterizations for the nanomaterial component of the sample derived from analytical techniques performed under in vitro conditions.
To add an in vitro characterization
...
Click the All tab or the In Vitro Characterizations tab and click Add next to In Vitro Characterization.
...
Both tabs provide customizations based on your Characterization Type* selection.
...
If you select one of the following characterization names, you need to fill in additional properties.
- Cytotoxicity
- Enzyme Induction
- Transfection
...
Add data findings and supporting documentation relating to the sample. Import as many .csv files as you wish or add derived data manually.
Click Add to expand the section, and add data findings and add supporting documents to the characterization.
...
When you are finished, click Submit to save the data to the sample.
...
If you selected one of the Characterization Names listed in the following table, the Properties section opens, and you must fill in additional information.
...
Cytotoxicity
...
Enzyme Induction
...
Enter the Enzyme Name of your choice.
...
Transfection
...
Enter the appropriate Cell Line.
...
In vivo characterization allows you to add characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vivo conditions.
To add an in vivo characterization
...
Click the All tab or the In Vivo Characterizations tab and click Add next to In Vivo Characterization.
Both tabs provide customizations based on your Characterization Type* selection.
Follow these steps to fill in the characterization. Links are provided for additional details.
...
Add data findings and supporting documentation relating to the sample. Import as many .csv files as you wish or add derived data manually.
Click Add to expand the section, and add data findings and add supporting documents to the characterization.
...
Select samples in the list to which you want this physico-chemical data transferred. This option copies files and data to one or more selected samples "owned" by the same point of contact.
...
When you are finished, click Submit to save the data to the sample.
...
To add an ex vivo characterization
...
Click the All tab or the Ex Vivo Characterizations tab and click Add next to Ex Vivo Characterization.
Both tabs provide customizations based on your Characterization Type* selection.
Follow these steps to fill in the characterization. Links are provided for additional details.
...
Add data findings and supporting documentation relating to the sample. Import as many .csv files as you wish or add derived data manually.
Click Add to expand the section, and add data findings and add supporting documents to the characterization.
...
Select samples in the list to which you want this physico-chemical data transferred. This option copies files and data to one or more selected samples "owned" by the same point of contact.
...
When you are finished, click Submit to save the data to the sample.
...
To add an other ex vivo characterization
...
Click the All tab or the Other Ex Vivo Characterizations tab and click Add next to Ex Vivo Characterization.
Both tabs provide customizations based on your Characterization Type* selection.
Follow these steps to fill in the characterization. Links are provided for additional details.
...
Add data findings and supporting documentation relating to the sample. Import as many .csv files as you wish or add derived data manually.
Click Add to expand the section, and add data findings and add supporting documents to the characterization.
...
Select samples in the list to which you want this physico-chemical data transferred. This option copies files and data to one or more selected samples "owned" by the same point of contact.
...
When you are finished, click Submit to save the data to the sample.
Adding Information to a Characterization
The following procedures apply to any Characterization Type when you are adding or editing a characterization.
...
When you are adding a characterization, fill in the following characterization information.
...
This is already filled in with Physico-Chemical, In Vivo, Ex Vivo, or Other Ex Vv (required) when you click the tab of the same name or add one from the All tab.
...
Select the name of the characterization (required).
| Info | ||
|---|---|---|
| ||
|
To create a new, non-standard Assay Type
- Select other.
- Enter a New Characterization Name and click Add, and the new characterization is added the Characterization Name list.
...
Select an assay type from the list that populates automatically based on the Characterization Name.
To add an Assay Type
- Select other.
- Enter a New Assay Type and click Add, and the new Assay Type is added to the list.
- Select other.
...
When you are adding a characterization, fill in the following design, methods, and techniques used.
...
Design and Methods Description
...
Enter a description for the characterization design and methods.
| Info | ||
|---|---|---|
| ||
If you include an extra line between text in the Characterizations free text, Design and Methods Description, the line separation is preserved when you save or update the characterization. |
...
Technique and Instrument
...
Technique*
...
When you are finished, click Save or Cancel to close the section without saving.
...
To add findings to a characterization, you can add data directly to Data and Conditions by creating columns and adding data manually or you can import as many .csv files as needed. Information can include laboratory conditions, pH, or temperature. You can add as many files as you wish.
...
- Save the spreadsheet of data values to a csv (comma-separated value) file.
- Click Import csv and select and follow the prompts to add the data file to the Findings Info.
- The columns and data are added to Data and Conditions.
...
| Info |
|---|
Whether you imported or added information manually, you can preface each data value with one of the following: Maintain the default, equal to (=), or select greater than (>), less than (<), or infinity (approximate). |
| Panel | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||
|
...
Select a Column Name or select other and add a new one.
| Info | ||
|---|---|---|
| ||
| You can add up to three cell viability Column Names, including cell viability, cell viability B, and cell viability C. You can further identify the column with the Column Value Type. |
For Column Type, Datum, the following characterization(s) display customized Column Name options.
...
- Molecular – Molecular Weight
- Purity – % purity for sample
- Relaxivity – R1, R2, T1, T2
- Size – PD1, Peak N , RMS size, Z Average
- Surface – charge, zeta potential
...
Enzyme Induction – % of Control
...
For Column Type, Condition, all characterizations provide the Column Name options in the left column of the following table. The Column Name autopopulates the Condition Property options in the right column.
...
...
N/A
...
...
media type, serum percentage
...
...
bandwidth, frequency, time, wavelength
...
...
N/A
...
...
lyophilized, time
...
...
time
...
...
N/A
...
...
N/A
...
...
lyophilized, time
...
...
ion concentration, ionic strength, molecular formula, osmolality, serum percentage, with serum
...
...
number of pulses, pulse duration
...
...
N/A
...
To further identify a column, select a Column Value Type.
| Info |
|---|
| Once the column information is saved, the Column Type is shown in parentheses after the Column Name, such as cell viability (mean). |
...
If you want the same value to fill all rows in a column, add a Constant Value.
| Info | ||
|---|---|---|
| ||
| For Column Value Type, boolean, enter a Constant Value of 1 for true and 0 for false. |
Click Save, and the column(s) are updated.
| Info |
|---|
If needed, click Set Column Order to change the order of the column headings in the matrix. |
...
...
To add results in the form of supporting documents, such as a graphic, spreadsheet or images to a characterization
| Tip |
|---|
You can add as many files as you wish. |
- In the Finding section of the characterization, next to Files, click Add.
- Upload, browse, and select the file or enter the file's URL where the document is located.
- Complete the following.
- Select the File Type (required), Document, Graph, Image, Movie, or Spreadsheet.
- Enter the File Title (required).
- Specify Keywords to associate with the file
- Enter a Description of additional information of the file.
Click Submit to add the file(s) to the sample.
...
The composition files and/or derived data for a sample can be copied to other samples from the same primary point of contact.
- From the All tab's characterizations summary, click Edit at the right of a characterization section.
- The Characterization opens and at the bottom of the page, in the Copy to other samples... section, select one or more samples with the same primary point of contact.
- Click Also copy finding data and conditions?.
Click Submit to copy the characterization.
Additional Characterization Tasks
The following table lists tasks that require Curator privileges.
| Info |
|---|
| If a button does not display, you have read-only access to the data. |
...
Printing Characterization(s)
...
To print the characterization(s), from the All tab characterization summary, click Print at the top right of the page.
...
Exporting Characterization(s) to a Spreadsheet
...
To export the characterization(s) to a spreadsheet, from the All tab characterization summary, click Export at the top right of the page.
| Info |
|---|
To export all sample information to JSON or XML, see Exporting Sample Information to JSON or XML |
...
Editing a Characterization
...
- From the All tab's characterizations summary, click Edit at the right of the characterization section you want to change.
- The Characterization opens and you can edit it as needed.
...
Deleting a Characterization
...
| Info | ||
|---|---|---|
| ||
This deletes a characterization(s) from the selected sample. If the characterization(s) was copied to other samples, the characterization is not removed from those samples. |
To delete a characterization for a selected sample
- From the All tab's characterizations summary, click Edit at the right of a characterization section.
- The Characterization opens, and click Delete.
- The deleted characterization is placed in the sample archive for historical purposes.
...
