{scrollbar:icons=false}

Page info
title title

This section describes how to review or ascribe characterizations to samples that have been submitted to caNanoLab. This section includes the following topics:

Understanding the Navigation Tree

Return to top of page

Anchor
Characterization Characterization

[Sample] Characterization Overview

Sample characterization, describing distinctive characteristics or essential features of the sample determined through analytical methods, records information associated with sample synthesis and properties. Samples can be characterized in caNanoLab by physical or chemical characteristics, or by data derived under in vitro and in vivo conditions.

• Physico-chemical characterization refers to the characterization of physical and chemical properties of a sample.
• In vitro characterization refers to characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vitro conditions.
• In vivo characterization refers to characterization for the nanomaterial component of the sample determined by the effect of samples on living cells.

In the Navigation Tree on the left sidebar, click the Characterization button. Tabs show characterization subcategories on the page that opens. Select the Add button on the All tab that corresponds to the category of characterization annotations you want to add, or select the tab itself where you can add characterizations to the sample. The All tab displays any characterizations that have already been added to the sample; the characterization category tabs also display annotations specific to those categories.

Return to top of page

Submitting Characterizations

The page that opens after you click an Add button to add a characterization is very similar for all characterization types. The page differs only in the Properties section if there are special properties driven by the selection of characterization names. They also differ in the drop-down values for the column name in the Finding section driven by the characterization name and assay type selections.

Follow the links below for more information about entering characterization information for the sample of interest.

• Adding a Physico-chemical Characterization
• Adding an In Vitro Characterization
• Adding an In Vivo Characterization

See also  Viewing Characterizations Summary.

Return to top of page

Anchor
PhysicoChemical PhysicoChemical

Adding a Physico-Chemical Characterization

 After you click the Characterizations button on the Navigation Tree, on the All tab click the Add button associated with Physico-Chemical Characterizations or select the Physico-Chemical Characterization tab where you can also click the Add button to add characterizations. The Physico-Chemical Characterization subcategory allows you to enter physical or chemical characterizations for the nanomaterial component of the sample.

The page that opens is organized into five sections. This page is the same for all physico-chemical characterizations, except for slight customizations based what you select in the Characterization Type* field. Instructions for using the page are outlined in the steps below.

1. The Characterization type is already selected as Physico-Chemical Characterization. In the Characterization Name field in the upper right, select from the drop-down list the name of the Characterization you want to add.
   For more information, see Defining Physico-Chemical Characterization.
2. The [Characterization] Properties section displays only for Physical State, Shape and Solubility characterizations. Define properties for the characterization, if applicable. For specific information, see Defining Physico-Chemical [Characterization] Properties.
3. In the Design and Methods section, complete the fields describing techniques and instruments used to derive the data. For more information, see Defining Physico-Chemical Design and Methods.
4. In the Finding section, add data findings and supporting documentation relating to the sample. For more information, see Describing Findings for Physico-Chemical Characterization.
5. In the Analysis and Conclusion section, enter any relevant analyses and conclusions reached by the data.
6. In the Copy to Other Samples... section, select samples in the list to which you want this physico-chemical data transferrred. This option copies files and data to one or more selected samples "owned" by the same point of contact. For more information, see Copying Characterizations.
7. When you finish, click Submit to save the data to the sample or click Reset to clear all fields on the form.

Return to top of page

Defining Physico-Chemical Characterization

To continue defining Physico-Chemical Characterization fields

1. The Assay Type field populates automatically with your selection in the Characterization field.
2. If the Protocol Name – Version field is available, select from the drop-down list the protocol from which the data is derived. A hyperlink of the protocol text file may appear. Click the hyperlink to open or save the file.
3. For Characterization Source, select from the drop-down list or enter the source from which the characterization data is derived, such as a vendor or a laboratory (NCL).
4. In the Characterization Date field, select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy.

After defining the summary, continue the process described in Adding a Physico-Chemical Characterization.

Anchor
Physico-Chemical Characterization Properties Physico-Chemical Characterization Properties

Defining Physico-Chemical [Characterization] Properties

The [Characterization] Properties section opens only for the characterizations listed in the table below. To define properties for the characterizations, complete the fields as described in the following table.

After defining the [characterization] properties, continue the process described in Adding a Physico-Chemical Characterization.

Return to top of page

Defining Physico-Chemical Design and Methods

In the Design and Methods sections, complete the fields as described in the following table.

Once you enter the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.

Continue the process described in Adding a Physico-Chemical Characterization.

Return to top of page

Describing Findings for Physico-Chemical Characterization

In the Finding sections, you can add one or more publications relevant to the sample as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.

Click Add to expand the section, then complete the instructions as described in Adding Data and Conditions and Adding a File.

Return to top of page

Adding Data and Conditions

In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information, such as laboratory conditions like pH or temperature that are part of your findings.

To define the data and condition matrix, follow these steps.

After adding data and conditions to the sample, continue the process described in Adding a Physico-Chemical Characterization.

Return to top of page

Adding a File

After adding one or more files, continue by Adding Data and Conditions or if you have no derived data to add, return to the steps described in Adding a Physico-Chemical Characterization.

Return to top of page

Anchor
InVitro InVitro

Adding an In Vitro Characterization

After you click the Characterizations button on the Navigation Tree, on the All tab click the Add button associated with In Vitro Characterizations or select the In Vitro Characterization tab where you can also click the Add button to add characterizations. The In Vitro Characterization subcategory allows you to add characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vitro conditions.

The page that opens is organized into five sections. This page is the same for all in vitro characterizations, except for slight customizations based what you select in the Characterization* field. Instructions for using the page are outlined in the steps below.

1. The Characterization type is already selected as In Vitro Characterization. In the Characterization Name* field in the upper right, select from the drop-down list the name of the Characterization you want to add (required).
   For more information, see Defining an In Vitro Characterization.
2. The [Characterization] Properties section displays only for Cytotoxicity, Enzyme Induction and Transfectionin vitro characterizations. Define properties for the characterization, if applicable. For specific information, see Defining In Vitro Characterization Properties.
3. In the Design and Methods section, complete the fields describing techniques and instruments used to derive the data. For more information, see Defining In Vitro Design and Methods.
4. In the Finding section, add data findings and supporting documentation relating to the sample. For more information, see Describing Findings for an In Vitro Characterization.
5. In the Analysis and Conclusions section, enter any relevant analyses and conclusions reached by the data.
6. In the Copy to Other Samples... section, select samples in the list to which you want this physico-chemical data transferrred. This option copies files and data to one or more selected samples "owned" by the same point of contact. For more information, see Copying Characterizations.
7. When you finish, click Submit to save the data to the sample or click Reset to clear all fields on the form.

Return to top of page

Defining an In Vitro Characterization

To continue defining this characterization

1. The Assay Type field populates automatically with options that display only for these in vitro characterization selections: Blood Contact, Cytotoxicity, Immune Cell Function, Oxidative Stress, Sterility and Targeting. For details about these options, see Assay Type Options for In Vitro characterizations. Select an option, if appropriate, or if there are none or if you prefer, select [Other] to name the assay type.
2. If the Protocol Name – Version field is available, select from the drop-down list the protocol from which the data is derived. A hyperlink to the protocol text file should appear (if the protocol is not private to the user and the protocol file exists). However, protocol files are not required. Click the hyperlink to open or save the file.
3. A hyperlink to the protocol text file should appear (if the protocol is not private to the user and the protocol file exists - ie. protocol files are not required).
4. Select from the drop-down list or enter the Characterization Source from which the characterization data is derived, such as a vendor or a laboratory (NCL).
5. In the Characterization Date field, select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy.

After defining the summary, continue the process described in Adding an In Vitro Characterization.

Return to top of page

Assay Type Options for In Vitro Characterizations

Specify one of the following in vitro Assay Type options or select [Other] to open a window where you can add a new assay type.

Return to top of page

Defining In Vitro [Characterization] Properties

The [Characterization] Properties section opens only when you select the following characterizations in the Summary section. To define properties for each unique characterization, enter information for the following fields.

• Cytotoxicity – Enter the appropriate Cell Line.
• Enzyme Induction – Enter your name of choice.
• Transfection – Enter the appropriate Cell Line.

After defining the [characterization] properties, continue the process described in Adding an In Vitro Characterization.

Return to top of page

Defining In Vitro Design and Methods

In the Design and Methods sections, complete the fields as described in the following table.

Once you enter the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.

Continue the process described in Adding an In Vitro Characterization.

Return to top of page

Describing Findings for an In Vitro Characterization

In the Finding sections, you can add one or more publications relevant to the sample as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.

Click Add to expand the section, then complete the instructions as described in Adding Data and Conditions and Adding a File.

Return to top of page

Adding Data and Conditions

In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information, such as laboratory conditions that are part of your findings.

To define the matrix for the data, follow these steps.

After adding data and conditions to the sample, continue the process described in Adding an In Vitro Characterization.

Return to top of page

Adding a File

Return to top of page

Anchor
InVivo InVivo

Adding an In Vivo Characterization

After you click the Characterizations button on the Navigation Tree, on the All tab click the Add button associated with In Vivo Characterizations or select the In Vivo tab where you can also click the Add button to add characterizations. The In Vivo Characterization subcategory allows you to add characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vivo conditions.

The page that opens is organized into five sections. This page is the same for all in vivo characterizations. Instructions for using the page are outlined in the steps below.

1. The Characterization type is already selected as In Vivo Characterization. In the Characterization Name field in the upper right, select from the drop-down list the name of the Characterization you want to add. The options for In Vivo Characterization are Pharmacokinetics and Toxicology. There are no customizations on this Characterization page based on either of these selections.
   For more information about completing the Summary section, see Defining an In Vivo Characterization Summary.
   No [Characterization] Properties section displays for in vivo characterizations.
2. In the Design and Methods section, complete the fields describing techniques and instruments used to derive the data. For more information, see Defining In Vivo Design and Methods.
3. In the Finding section, add data findings and supporting documentation relating to the sample. For more information, see Describing Findings for In Vivo Characterizations.
4. In the Analysis and Conclusion section, enter any relevant analyses and conclusions reached by the data.
5. In the Copy to Other Samples... section, select samples in the list to which you want this physico-chemical data transferrred. This option copies files and data to one or more selected samples "owned" by the same point of contact. For more information, see Copying Characterizations.
6. When you finish, click Submit to save the data to the sample or click Reset to clear all fields on the form.

Return to top of page

Defining an In Vivo Characterization Summary

To continue defining Summary fields

1. You can leave the Assay Type field blank or specify an Assay Type.
2. If the Protocol Name – Version field is available, select from the drop-down list the protocol from which the data is derived. A hyperlink of the protocol text file may appear. Click the hyperlink to open or save the file.
3. Select from the drop-down list or enter the Characterization Source from which the characterization data is derived, such as a vendor or a laboratory (NCL).
4. In the Characterization Date field, select from the calendar or enter the date the characterization was made. Acceptable format: dd/mm/yyyy.

After defining the summary, continue the process described in Adding an In Vivo Characterization.

Return to top of page

Defining In Vivo Design and Methods

In the Design and Methods sections, complete the fields as described in the following table.

Once you specify the data, click Save to save the information to the sample or Cancel to close the mini-window without saving data.

Continue the process described in Adding an In Vivo Characterization.

Return to top of page

Describing Findings for In Vivo Characterizations

In the Finding sections, you can add one or more publications relevant to the sample as well as data derived for the sample. You can add as many files and derived data as you wish, or you can add derived data without adding files.

Complete the instructions as described in Adding Data and Conditions and Adding a File.

Return to top of page

Adding Data and Conditions

In the Data and Conditions segment of the window, you can create a matrix where you can enter data values and/or other information that are part of your findings.

To define the matrix for the data

For an in vivo characterization, click [Other] to name the column yourself.

Return to top of page

Adding a File

After adding one or more files, continue by Adding Data and Conditions or if you have no derived data to add, return to the steps described in Adding an In Vivo Characterization.

Return to top of page

Viewing Characterizations Summary

Sample characterization, describing distinctive characteristics or essential features of the sample determined through analytical methods, records information associated with sample synthesis and properties. Samples can be characterized in caNanoLab by physical or chemical characteristics, or by data derived under in vitro and in vivo conditions.

• Physico-chemical characterization refers to the characterization of physical and chemical properties of a sample.
• In vitro characterization refers to characterizations for the nanomaterial component of the sample that were derived from analytical techniques performed under in vitro conditions.
• In vivo characterization refers to characterization for the nanomaterial component of the sample determined by the effect of samples on living cells.

To view a sample characterizations summary

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations link. Five tabs showing characterization subcategories display on the page that opens.
3. The All tab displays characterizations that have already been added to the sample by category with anchor links to each. In addition, the characterization category tabs also display corresponding annotations.

caNanoLab lists characterizations that have been added to a sample under their corresponding Physical, In Vitro and In Vivo Characterization subcategories when you select the Characterization button on the Navigation Tree.

See also Characterization Tasks.

Return to top of page

Characterization Tasks

From a characterization summary page, and with curator privileges, you can perform these tasks:

• Printing a Characterization
• Exporting Characterizations
• Editing Characterizations
• Copying Characterizations
• Deleting a Characterization

Return to top of page

Printing a Characterization

To print a characterization

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations button. The All tab displays the characterizations summary.
3. Click the Print hypertext link at the top right of the page.

Return to top of page

Exporting Characterizations

To export a characterization

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations button. The All tab displays the characterizations summary.
3. Click the Export hypertext link at the top right of the page.

Return to top of page

Editing Characterizations

To edit a characterization

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations button. The All tab displays the characterizations summary.
3. Click the Edit hypertext link at the right of the characterization section you want to change. This opens the corresponding Characterization page where you can edit the file by following the same directions as described for creating characterizations.

Return to top of page

Copying Characterizations

The characterization files and/or derived data for this sample can be copied to other samples from the same primary point of contact.

To copy characterizations, open the sample characterization form that displays the characterization

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations button. The All tab displays the characterizations summary.
3. On the characterization summary page, click the Edit hypertext link at the right of the section you want to copy. This opens the corresponding Characterization page.
4. At the bottom of the page, in the Copy to other samples... box, select one or more samples that have the same primary point of contact (those listed).
5. Click the Also copy characterization results? box.
6. Click Submit to execute the copy function.

Return to top of page

Deleting a Characterization

To delete a characterization for a selected sample

1. Open the sample of your choice.
2. In the Navigation Tree on the left sidebar, click the Characterizations button. The All tab displays the characterizations summary.
3. Click the Edit hypertext link at the right of the section you want to delete. This opens the corresponding Characterization page.
4. Click the Delete button at the bottom left of the page.

Deleted characterizations are placed in the sample archive for history purposes.

Return to top of page

Publication Tasks Associated with a Sample

Click the Publications button on the Navigation Tree. A Publication[s] summary page displays those publications associated with the sample, and the publication identifier, title, authors and year, where appropriate. Additionally, each publication category tab displays its corresponding publications.

Either on the Publications All tab or the subcategory tabs, you can

• Access a publication, click the Abstract/Download hypertext link. This opens the document.
• Add a publication to the open sample, click the Add button corresponding to the publication type on the All tab or select a publication type tab and click the Add button. This opens the Submit Publication page identical to that opened from the Publications menu option with one exception:

If you work from the Publications menu option, you can associate multiple samples with a publication. Alternatively, if you add publications to an open sample using the Navigation Tree option, you can use the copy feature to associate the publication with other samples in the same way you copy characterizations to multiple samples. See Copying Characterizations.

Return to top of page

{scrollbar:icons=false}