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You must have all your experiment data readily accessible on your computer (i.e., not archived or compressed). The data should preferably be consolidated into a single location (i.e., a folder containing every single IDF, SDRF, TXT, and TSV file from the experiment). You will also need an archive creation utility installed on your computer. In this tutorial, we will use WinZip (www.winzip.com), but any comparable utility with support for the ZIP format will do.
Reference Information
The experimental experiment data used in this tutorial was not generated de novo; it came from an existing experiment whose data is publicly available on the official NCI instance of caArray at https://array.nci.nih.gov/caarray/home.action (you must have an official NCI user account to access this site). The experiment, entitled "TCGA Ovarian: Comparative Genome Hybridization Analysis Using the Agilent Human Genome CGH 244A Platform", was conducted at Harvard Medical School in Boston, MA. It can be accessed via the URL https://array.nci.nih.gov/caarray/project/EXP-498 or by searching for the experiment ID 'EXP-498' on the NCI caArray instance. The array design used was TCGA-Agilent_HG-CGH-244A; the ADF array design files can be downloaded from the experiment, as can all the experiment data, including the IDF and SDRF metadata files, the Agilent TXT raw array data files, and the TSV derived array data files.
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In preparing your data for upload, the first step is to find all the files associated with a given IDF file. To so, open any of the IDF files from your experiment in Microsoft Excel or another application suited for viewing tab-limited data. The partial screenshot below shows the first of twelve IDF files from our example experiment as viewed in Excel.
The field 'SDRF files' towards the bottom of your IDF file displays the name of the SDRF file that is associated with the IDF.
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