Overview
caIntegrator provides the capability to perform level 3 copy number analysis using either caDNAcopy or caCGHcall. Both of these tools are part of the Bioconductor suite and are implemented as caGrid services wrapping the R Bioconductor code. One or both of these tools can be installed either on a separate server from caIntegrator or on the same server. Be aware that the R processing associated with caDNAcopy and/or caCGHcall can be very CPU and memory intensive. Also, the current installation instructions only support installation on Linux platforms. Recommended configurations are given at the bottom.
Prerequisites
The following software packages must be installed prior to beginning the instructions:
- Java Development Kit (JDK) 1.6.x
- Ensure that JAVA_HOME is defined, preferably in the user or system profile
- Ensure that $JAVA_HOME/bin is in PATH
- Ant 1.7.x
- Ensure that ANT_HOME is defined, preferably in the user or system .profile
- Ensure that $ANT_HOME/bin is in PATH
- Tomcat 5.5.x
- Instructions assume Tomcat is configured to run on port 8080, if you are using another port, please substitute it into later instructions
- Ensure that CATALINA_HOME is set
- Tomcat may be autostarted or started as part of the Bioconductor launch script (provided later in these instructions)
Install Dependent Packages
The caIntegrator Bioconductor installation also depends on several other packages that may not be installed on your system. Summaries of these packages and how to install them follow.
Globus 4.0.3
Installed at /usr/local/ws-core-4.0.3
- Download from http://www-unix.globus.org/ftppub/gt4/4.0/4.0.3/ws-core/bin/ws-core-4.0.3-bin.tar.gz
- Unpack tar file into /usr/local/ws-core.4.0.3
- Ensure that GLOBUS_LOCATION is set to /usr/local/ws-core-4.0.3
Apache Axis 1.4
Installs files into $CATALINA_HOME
- Download from http://apache.cs.utah.edu/ws/axis/1_4/axis-bin-1_4.tar.gz
- Unpack tar file in home directory
- Move axis-1_4/webapps/axis directory to $CATALINA_HOME/webapps
- Stop and restart Tomcat ($CATALINA_HOME/bin/shutdown.sh; $CATALINA_HOME/bin/startup.sh)
- You can test Axis installation at http://YOUR-HOSTNAME:8080/axis/happyaxis.jsp
ActiveMQ 4.0.2
Installed at /usr/local/incubator-activemq-4.0.2
- Download from http://people.apache.org/repository/incubator-activemq/distributions/incubator-activemq-4.0.2.tar.gz
- Unpack tar file into /usr/local/incubator-activemq-4.0.2
- Ensure that JMS_HOME is set to /usr/local/incubator-activemq-4.0.2
- Edit file $JMS_HOME/conf/activemq.xml
- Change line that reads "<broker useJmx="true">" to "<broker useJmx="true" persistent="false">"
- Replace line that reads "<transportConnector name="default" uri="tcp://localhost:61616" discoveryUri="multicast://default"/>" with " <transportConnector name="default" uri="tcp://localhost:61616"/>"
- Comment out or remove line that reads "<networkConnector name="default" uri="multicast://default"/>"
- Make main binary executable
- chmod 755 $JMS_HOME/bin/activemq
- ActiveMQ may be autostarted or started as part of the Bioconductor launch script (provided later in these instructions)
R 2.9.0
Installed at /usr/local/R-2.9.0
- Download from http://cran.fhcrc.org/src/base/R-2/R-2.9.0.tar.gz
- Unpack tar file in home directory
- Configure, build, and install
- cd R-2.9.0
- ./configure --enable-R-shlib --with-readline=no --with-x=no
- Fix src/modules/Makefile as follows (fix is from http://tolstoy.newcastle.edu.au/R/e6/devel/09/04/1434.html)
- Change lines that read:
for d in "$(R_MODULES)"; do \ (cd $${d} && $(MAKE) $@) || exit 1; \ done
- To read:
@if test "$(R_MODULES)" != ""; then \ for d in "$(R_MODULES)"; do \ (cd $${d} && $(MAKE) $@) || exit 1; \ done; \ fi
- Be very careful of tabs in the lines, one tab to start each line, indent beyond that with spaces. Be careful to have no spaces at the end of the lines too. Use rest of Makefile as an example.
- Change lines that read:
- make
- make install prefix=/usr/local/R-2.9.0
Dependent R packages (RCurl, SJava, RWebServices, RUnit, DNAcopy)
Installs files into $R_HOME
- $R_HOME/bin/R
- At the R prompts:
- source("http://www.bioconductor.org/biocLite.R")
- biocLite()
- biocLite("RCurl")
- biocLite("SJava")
- biocLite("RWebServices")
- biocLite("RUnit")
- biocLite("DNAcopy")
- q()
- Build SJava links missed by install:
- cd $R_HOME/lib64/R/library/SJava/libs
- ln -s SJava.so libRInterpreter.so
- ln -s SJava.so libSJava.so
- Build and test SJava/RWebServices installation (as per Bioconductor Installation Guide page 10)
- $R_HOME/bin/R
- At the R prompts:
- library(RWebServices)
- unpackAntScript("/tmp/rservices")
- q()
- cd /tmp/rservices
- ant recompile-sjava
- ant basic-prop
- Look for any errors)
- ant rservices-test
- This step is optional
Install caDNAcopy
This section installs the caDNAcopy grid in three pieces: base code, R web service, and caGrid service. You may skip this section if you are only using caCGHcall.
caDNAcopy Base Code
Installs into R_HOME
- cd to your home directory
- svn checkout http://gforge.nci.nih.gov/svnroot/bioconductor
- cd bioconductor/trunk/services/caDNAcopy/R
- $R_HOME/bin/R CMD INSTALL caDNAcopy
caDNAcopy RWebService
Installs into /usr/local/bioconductor/caDNAcopy
- cd /usr/local/bioconductor
- $R_HOME/bin/R
- At the R prompts:
- library(RWebServices)
- unpackAntScript("caDNAcopy")
- q()
- cd caDNAcopy
- ant map-package -Dpkg=caDNAcopy
- ant unpack-package -Dpkg=caDNAcopy
- ant precompile
- Optionally, test RWebService (as per Bioconductor Installation Guide page 11):
- Start up Tomcat (shutdown if already running)
- $CATALINA_HOME/bin/startup.sh
- Start up ActiveMQ
- $JMS_HOME/bin/activemq &
- Start up R worker for caDNAcopy
- nohup ant start-worker &
- Run the test
- ant local-test
- Check any failures in test/output. Ignore failures that say "expected 79, got 80"
- Start up Tomcat (shutdown if already running)
caDNAcopy Grid Service
Installs into $CATALINA_HOME
- Download Bioconductor-caGrid-Services.tar.gz from https://gforge.nci.nih.gov/docman/view.php/175/19977/Bioconductor-caGrid-Services.tar.gz
- Unpack tar file in home directory
- Build and deploy caDNAcopy grid service
- cd caGrid/CaDNAcopy
- ant deployTomcat
- Prepare Tomcat for Grid applications
- cd $GLOBUS_LOCATION
- ant -f share/globus_wsrf_common/tomcat/tomcat.xml deployTomcat -Dtomcat.dir="$CATALINA_HOME"
- Copy $JMS_HOME/lib/*.jar to $CATALINA_HOME/webapps/wsrf/WEB-INF/lib
- Optionally, fix timeout value in caDNAcopy.jar to be 2 hours instead of 60 seconds
- mkdir ~/unjar; cd ~/unjar
- unzip /usr/local/tomcat-5.5.27-8080/webapps/wsrf/WEB-INF/lib/caDNAcopy.jar
- Edit org/bioconductor/rserviceJms/services/caDNAcopy/RWebServices4java.properties
- Change to: jms.timeout=7200000
- zip -r caDNAcopy.jar *
- cd /usr/local/tomcat-5.5.27-8080/webapps/wsrf/WEB-INF/lib
- cp ~/unjar/caDNAcopy.jar .
- Optionally, fix timeout value in R worker to be 2 hours instead of 60 seconds
- Edit $R_HOME/lib64/R/library/RWebServices/scripts/RWebServicesTuning.properties
- Change to: jms.timeout=7200000
- Edit $R_HOME/lib64/R/library/RWebServices/scripts/RWebServicesTuning.properties
Launch Bioconductor Services
This section describes how to start up all the pieces of the Bioconductor installation once they have been successfully installed. It also contains optional scripts that can automate the startup and shutdown of all processes.
Starting Bioconductor Services
As per Bioconductor Installation Guide page 11:
- Start up Tomcat (shutdown if already running)
- $CATALINA_HOME/bin/startup.sh
- Start up ActiveMQ
- $JMS_HOME/bin/activemq &
- Start up R worker for caDNAcopy (skip if only using caDNAcopy)
- cd /usr/local/bioconductor/caDNAcopy
- nohup ant start-worker &
- Start up R worker for caCGHcall (skip if only using caCGHcall)
- cd /usr/local/bioconductor/caCGHcall
- nohup ant start-worker &
Scripts to startup/shutdown Bioconductor
These shell scripts can be used to automate the launch of Bioconductor. If these are used to automatically start the Bioconductor services at system startup, please ensure that all environment variables (as listed in sections above) are already defined.
- start-bio.sh
- stop-bio.sh