NIH | National Cancer Institute | NCI Wiki  

Error rendering macro 'rw-search'

null

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 6 Next »

caMicroscope is a tool to view, annotate, and analyze biomedical images. It consists of a set of web services to manage digital pathology images, associated clinical and imaging metadata, and human/machine generated annotations and markups. It also allows you to view and analyze whole-slide and nuclear segmentations of images. caMicroscope hosts whole-slide, cancer tissue images for review by pathologists.

FeatureScape provides interactive representations of feature landscapes. Use FeatureScape to explore slide-level imaging features generated by analysis of whole slide tissue images to QuIP.

This guide explains how to do the following tasks in caMicroscope:

Opening caMicroscope

  1. Use the Chrome browser to navigate to https://wolf.cci.emory.edu/vtr_pending.
    The Sign In page appears.
  2. Use your Google credentials to sign in to caMicroscope.
    The caMicroscope site appears.
  3. Select which database of images you want to view by clicking either the large slide image or the More button. Databases include Breast Cancer Genomic Pilot, Virtual Tissue Repository (VTR) Pending, and Pancreatic Ductal Adenocarcinoma (PDAC).
    A table appears that lists all whole-slide images for the selected database that you are authorized to view.
  4. In the table, click the Open button for any row.
    The slide opens in caMicroscope.

    To select a different image, click the Back button in your browser to return to the table.

Using the caMicroscope Toolbar

Use the toolbar buttons to manipulate the slide.

Tool

Name

Purpose


Home

Return to the caMicroscope home page.










Zooming In and Out

To zoom in on the image, click the main content window with your mouse. Each time you click, the view zooms in further, the scale updates, and the bounding box in the inset window changes to show how much of the total image is in the main content window. When you are zoomed in very far, the bounding box may not be visible. Zooming out reverses this process.

To Zoom In: Click in the main window.

To Zoom Out: Press and hold the Shift key while you click on the main window.

Zoomed in:


Zoomed out:


 

Panning an Image

Panning allows you to move the image inside the window.

  1. Select an image and view it in caMicroscope.
  2. In the inset window, note the red bounding box. This bounding box is your view of the current image in the main content window.
  3. Click your mouse to control the bounding box and drag it up, down, left, and right to see different parts of the main content window.

Using the Lymphocyte & Plasma Cell Annotation App

From caMicroscope you can launch the Lymphocyte & Plasma Cell Annotation App by clicking  on the caMicroscope toolbar. This app allows you to view and modify annotations related to lymphocyte and plasma cells.

The following tools are available on the toolbar.


Tool

Name

Purpose


Home

Return to the caMicroscope home page


caMicroscope

Return to the caMicroscope application


Filter Markups

View only those markups associated with selected algorithms. For more information, see page 8.


Show/Hide Markups

View the image with or without markups.


Decrease Opacity

Decrease the opacity of the selected markup(s), making it/them more transparent.


Increase Opacity

Increase the opacity of the selected markup(s), making it/them less transparent.


Show Weight Panel

This panel allows you to adjust the automatic lymphocyte prediction results for this slide, with the options described below.

  • Lymphocyte Sensitivity bar:

Adjust the sensitivity of lymphocyte prediction.

Choose a higher volume for more predicted lymphocyte regions.

  • Necrosis Specificity bar:

Adjust the specificity of necrosis prediction.

Choose a higher volume for more predicted lymphocyte regions.

  • Lymphocyte Prediction box:

Show lymphocyte prediction without necrosis filtering.

  • Necrosis Prediction box:

Show necrosis prediction.

  • Lym Prediction with Nec Filtering box:

Show lymphocyte prediction with necrosis filtering (recommended).


Free Line Markup

Draw thin lines, thick lines, or polygons on the image.


Switch User

If you are a super user, click this button to review and change other people’s annotations.

Using the Segment Curation App

Use the Segment Curation App to curate segmentation results.

The Segment Curation App has the following tools.


Tool

Name

Purpose


Home

Return to the caMicroscope home page.


Free Line Markup

Draw thin lines, thick lines, or polygons on the image.


Save ViewPort

For a selected algorithm, save the ViewPort.


Save Rectangle and Delete Annotations in This Area

For an area based on a selected algorithm, save the rectangle and delete the annotations.


Filter Markups

View only those markups associated with selected algorithms. For more information, see page 8.


Generate Composite Dataset



ColorMap


Filtering Markups

You can filter your view of the markups on the images in caMicroscope by an area based on one or more selected algorithms. The algorithms available in caMicroscope have been run against the full set of images.

To filter markups

  1. Click the Filter Markups button.
    The Select Algorithm box appears.
  2. Click the box to the left of each algorithm you want to select.
    caMicroscope runs the algorithm(s) against each image in the dataset. This can take some time. The markups appear in the color noted in the Select Algorithm box. You may have to zoom in and pan the image to see all markups.

Analyzing Images

To analyze images

  1. Log in to caMicroscope and then click the icon for FeatureScape.

    The SlideSelect page appears.
  2. From the drop-down list, select an analysis set containing images you want to view and explore. The analysis sets are analyses that have been run on the entire dataset. The diagnostic images within an analysis set have been analyzed with the algorithm named in the analysis set.
  3. Click a link in the caMicroscope column to view the diagnostic image in caMicroscope.
  4. Click the Explore Features button in the row of an image you want to explore.

    A plot of cross-tabulated feature correlations appears.

    NOTE: The heat map shown in the cross-tabulation is a sampling of over a million segmented objects in the dataset.
  5. Hover your mouse over the colored pixels in the feature correlations. Each pixel is a single segmented object. The features that intersect at each pixel are highlighted in yellow, the intersected pixel is outlined in orange and filled with an X, and the feature names appear in a popup window.

    NOTE: The colors in the heat map show how high or low the correlations between intersecting features are. The orange shows the pixels of highest correlation. The orange diagonal line shows where features are identical on both axes.

Viewing Scatterplots

To the right of the feature correlation plot is a scatterplot based on the computed feature values. The scatterplot shows how objects are distributed. Zoom in by selecting a region to look at the distribution in more detail.

When you move your cursor over the scatterplot, you see tools you can use in it. Hover your mouse over a tool to view the tool name.

Selecting a Region on the Scatterplot

Select a region on the scatterplot to zoom in to a subset of segmented objects.

To select a region:

  1. Move your cursor over the scatterplot to see the crosshairs.
  2. Click an area of interest and drag your cursor to create a rectangle.
  3. Release the mouse. The scatterplot zooms in to the selection.

You can now view nuclear mugshots or download data from the selected region. To return to the original scatterplot, click the selected pixel in the heat map again.

Viewing Nuclear Mugshots

Nuclear mugshots are screenshots of segmented nuclei in the set of features you selected in the heat map. Only a sampling of the millions of images has associated mugshots.

To view nuclear mugshots:

  1. Select an area of interest on the scatterplot.
  2. Click Nuclear mugshots from selected region.
    The nuclear segmentation viewer opens, showing tiles of slides that match the range you selected.
  3. Click any tile to open it in caMicroscope and view it in the context of the whole slide image.
    NOTE: Make sure you are logged into caMicroscope before you select a tile.

Downloading Data from a Scatterplot

To download data from a scatterplot

  1. Select an area of interest on the scatterplot.
  2. Click .
    The FeatureScape Data Download page appears.
  3. Review the information on the page to confirm the relative time it will take to download your selection.
  4. Enter the maximum number of features to download and then click .
    The Data Download page refreshes with a list of variables represented in the download. You can select some or all of them and then click .

Alternatively, click Save (partial) File as CSV. This skips the step of selecting individual variables.

  1. If you have selected variables, click  again.
  2. Click  to save the data in comma-separated value format in Chrome.
  • No labels