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(1) The Material file describes the composition of the material formulation and is not included in the ISA-TAB specification. The Material file is an important file that is designed to allow for nanomaterial comparison across nanotechnology resources.

(2) An investigation typically involves the preparation of multiple materials that are tested.These materials could be of the same chemical composition (same type and amount of chemical components in the sample) or different chemical compositions. Materials of different chemical composition or physical characteristics should be described in separate Material files.

File Format

The Material file looks similar to the Study and Assay files. It is a horizontally oriented spreadsheet containing tab delimited row values representing multiple values. The Material file contains information on the material, the relationship between material constituents, and a description of the material file. An example subset of this format is provided in TABLE 1.

TABLE 1: Example Subset of the Material File Format





Material Source Name

Material Name

 Manufacturer Lot Identifier

Material Description




G4 tris(hydroxyl) terminated PAMAM dendrimer





A detailed example leveraging data from the Nanotechnology Characterization Laboratory (NCL) NCL200612 is provided on the Material File Examples wiki page.



Material source name--- The unique identification name of the source from which the material sample is derived. Its value is used as the value for source name in ISA-TAB-Nano study files, thereby linking the ISA-TAB-Nano Material file and the Study file.

Material name---The unique identification name for the sample and its different components. This name is used to identify or reference the different components across the ISA-TAB-Nano files.

Manufacturer lot identifier---A distinctive numeric, alpha, or alpha-numeric identification code assigned by the manufacturer or distributor. It is assigned to a specific quantity of manufactured material or product that is produced in a manner that is expected to render it homogeneous.

Material description---A textual description of the material sample.

Material synthesis---A text or a single term description of how the material was made.

Material design rationale---A text description for the underlying design rationale is the property, process or phenomenon taken into consideration when formulating a nanoparticle or other substance in order to achieve the intended use of the formulation.

Attributes of Material Nodes

Material intended application---The application for which a drug, nanoparticle or other substance is formulated and tested (e.g., MRI).

Material type---One or more terms used to classify the type of material sample. Multiple terms are entered as a semicolon-delimited list.

Material chemical name---The chemical name of the material or its constituent material.

Characteristics--- An attribute of the sample and its constituents. The term for each characteristics [ ] attribute is written within brackets (for example, Characteristics [wall type], etc.). Nominal particle characteristics (or vendor supplied) should be included in the Material File as characteristics. Experimentally determined characteristics should be included in the Assay File.

Unit--- The standard of measurement used if the value for material characteristic value is quantitative and dimensional.

Material consitituent--- The material name for each of the components within a material. The names should be obtained from the entries for the “Material Name” field. If there is a "Material Linkage Type", then only the two linked components should be specified.

In the example below, Material M is comprised of A, B, and C and within Material M, A and B are linked and A and C are linked.

Material Source NameMaterial NameMaterial ConstituentMaterial Linkage Type
M-lab-1MA;Bcovalent linkage
M-lab-1MA; Ccovalent linkage

Material linkage type---The type of linkage present in a nanoparticle sample (for example, attachment, encapsulation, entrapment etc.). If the linkage type is an entrapment or encapsulation, the material type column can specify whether the constituent is entrapped or encapsulated 

Material data file --- The name of files (e.g. Image, Structures file) containing information about the material sample. There can be only one file name per cell.

Material data file type--- The name that defines the type of the material file (e.g. image, graph).

Material data file description--- A textual description providing additional information on the material file.

Qualifiers for Material Nodes’ Attributes

Each node attribute may be qualified using the following concepts, if applicable.

Unit---The standard of measurement used if the values for characteristic [ ] columns are quantitative and dimensional. If the term for unit is taken from an ontology/controlled vocabulary, then its term accession number and the term source REF should be defined in the ISA-TAB-Nano Material File.

Term Accession Number---Identification number of a term selected from an ontology or a controlled vocabulary, if the term is entered as a value in material intended application, material type, material chemical name, characteristic [ ], material linkage type [ ], material data file type [ ], unit or factor value [ ] columns.

Term Source REF---The name which identifies the source from where a term is selected and entered in ISA-TAB-Nano study files. This name should match one of the names entered in the term source name field in the ISA-TAB-Nano Investigation File.


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