The Assay File will vary depending on the type of assay performed and protocol and technology type leveraged. Provided below are examples of Assay Files for common types of assays identified across nanotechnology resources.
Size Assay
Size by Dynamic Light Scattering (DLS) Assay
Dynamic Light Scattering (DLS) is a type of spectroscopy that uses a laser beam to irradiate a sample containing particles in suspension resulting in light scattering. Rapid fluctuations in scattering intensity around a mean value at a certain angle occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusion coefficient, for a given temperature and viscosity, that can be used to calculate particle size.
| Assay File | Assay Factors | Assay Measured Values | Supporting Information |
|---|---|---|---|
| Size by DLS |
Cytotoxicity Assay
Hepatocarcinoma Cytotoxicity MTT Assay
The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. The Assay File represents an example for a MTT cytoxicity assay (MTT) performed on three nanoparticle samples, where 9 dilutions of each nanoparticle sample are exposed to porcine proximal tubule cells for three different times of exposure (6h, 2h, and 48 h).
| Assay File | Assay Factors | Assay Measured Values | Supporting Information |
|---|---|---|---|
| Hep G2 Hepatocarcinoma Cytotoxicity Assay |
