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At the time of writing of this document the curation in ISA-TAB-Nano follows the ISA-TAB-Nano 1.3 Release.  More detailed information is provided in ISA-TAB-Nano wiki  https://wiki.nci.nih.gov/display/icr/isa-tab-nano. The ISA-TAB-Nano forms filenames comprise a prefix corresponding to a specific form, i.e. i_ for an investigation form, s_ for a study form, a_ for an assay form, m_ for a material form, a custom abbreviation of institution(s) names, a name of the first author (first name abbreviation, full last name), a custom abbreviation of journal title and a year of publication, e.g. i_USC_UV-JCrecente-CampoJCR2019.  A suffix for Study indicates a study type e.g. physicochemical, in_vitro. A suffix for Assay file in addition to a type of Study is related to, includes a name of assay, e.g. size, zeta potential.

For example:

a_ USC_UV-JCrecente-CampoJCR2019-physicochemical-size-DLS.

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Figure 13. An Ontology Source Reference section in Investigation form

Enter the Investigation information into the Investigation form.

  • Investigation Identifier, in most cases, comprising institution names, first author, journal title, and publication year.
  • Custom investigation title, e.g. a rephrased publication title.
  • Custom investigation description, e.g. an abbreviated abstract.
  • Custom investigation outcome.
  • PubMed ID.
  • Publication DOI.
  • Author list.
  • Publication title.
  • Publication status (published, submitted, in press, in preparation).

INVESTIGATION


Investigation Identifier

USC_UV-JCrecente0CampoJCR2019

Investigation Title

Dependence of interaction with macrophages and biodistribution in zebrafish on size and composition of polymeric nanocapsules

Investigation Description

This work aimed to understand
the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo. A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (~ 70 nm) and medium (170–250 nm).

Investigation Submission Date


Investigation Public Release Date


Investigation Disease


Investigation Disease Term Accession Number


Investigation Disease Term Source REF


Investigation Outcome

The in vitro results showed that small nanocapsules interacted more efficiently with macrophages than their larger counterparts. Inulin nanocapsules were significantly less toxic than chitosan nanocapsules. Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small nanocapsules, regardless of their composition, disseminated considerably faster and further than their medium size counterparts. These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.

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  • Enter to a first column Material Source Identifier, i.e. a sample name as generated in section Data Extraction, e.g. USC_UV-JCrecente-CampoJCR2019-04
  • Enter a material name, e.g. core/shell_iron//iron_oxide into Material Name field.
  • Enter Manufacturer Lot Identifier, if is available.
  • Enter into Material Description a sample description.
  • Enter a description of sample synthesis into Material Synthesis field.
  • Enter a description of rationale to design specific sample into Material Design Rationale.
  • Enter Material Intended Application, its Term Accession Number, and Term Source REF from Bioportal.
  • Enter material type, e.g. nanoparticle sample, iron nanoparticle, into Material Type field. If several types are assigned to a sample, separate types by semicolon. Enter corresponding Term Accession Number and Term Source REF separated by semicolon.
  • Enter sample properties, e.g. molecular weight, into Characteristics/Material Characteristic field.
  • In the next lines, submit information about sample components, as above.
  • Enter chemical name of sample component into Material Chemical Name field, corresponding Term Accession Number from Bioportal, and Term Source REF. Check if the Ontology with this Term Accession Number is present in the Ontology Source Reference in the Investigation form (Figure 13). If it is not, then add this Ontology. Perform this process for all other forms and other Term Accession Numbers.
  • Enter into Characteristics fields additional information this particular constituent of sample, like amount, functionality, or molecular formula.
  • If amount of constituent is specified, enter unit into Unit field and corresponding Term Accession Number from Bioportal and Term Source REF.
  • If any two or more components of sample are linked, then in the very first line of Material form, enter Material Names of components, which are linked, into Material Part Name, and add information about a type of association into Material Linkage Type. Enter Term Accession Number corresponding to Material Linkage Type, and its Term Source REF (Figure 20). Repeat this step if the additional components are linked.

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