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Problem: An overview of finding information on an experiment in caArray.

Topic: caArray Usage

Release: caArray 2.0 and above

Date entered:

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10/12/2011

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Solution

A caArray experiment captures as much relevant information about a real-world

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experiment

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as

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possible.

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This

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can

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include

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general

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information

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about

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the

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experiment,

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such

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as

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the

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experimental

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design

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and

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experimental

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factors,

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associated

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publications,

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protocols,

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array

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designs,

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quality

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control

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and

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data

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processing

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steps,

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or

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specific

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information

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about

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biological

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materials

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used

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in

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the

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experiment.

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Files

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containing

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data

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generated

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for

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an

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experiment

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can

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be

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uploaded,

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validated

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and

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imported

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into

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a

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caArray

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experiment.

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Once

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a

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user

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clicks

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on

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an

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experiment

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at

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the

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caArray

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web

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interface,

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five

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main

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menu

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items

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are

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presented

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for

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each

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experiment:

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Overview,

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Contacts,

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Annotations,

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Data,

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and

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Publications.

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  • Overview:

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  • This

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  • page

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  • highlights

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  • several

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  • key

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  • characteristics

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  • of

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  • the

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  • experiment.

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  • The

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  • information

...

  • on

...

  • the

...

  • "Overview"

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  • page

...

  • either

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  • comes

...

  • from

...

  • manual

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  • data

...

  • entry

...

  • when

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  • the

...

  • experiment

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  • was

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  • created

...

  • or

...

  • comes

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  • from

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  • the

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  • loading

...

  • of

...

  • a

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  • MAGE-TAB

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  • IDF

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  • file.

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  • A

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  • few

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  • elements

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  • are

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  • worth

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  • mentioning:

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    • Experimental

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    • Description:

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    • a

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    • detailed

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    • description

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    • of

...

    • the

...

    • experiment.

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    • You

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    • can

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    • put

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    • as

...

    • much

...

    • details

...

    • as

...

    • you

...

    • want

...

    • but

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    • please

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    • be

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    • aware

...

    • that

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    • it

...

    • is

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    • just

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    • one

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    • single

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    • paragraph.

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    • Status:

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    • either

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    • "In

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    • Progress"

...

    • (default)

...

    • or

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    • "Locked"

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    • (the

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    • experiment

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    • cannot

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    • be

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    • edited)

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    • Array

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    • Design:

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    • this

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    • represents

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    • the

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    • file(s)

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    • describing

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    • probes

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    • on

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    • the

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    • corresponding

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    • microarray

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    • platform

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  • Contact:

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  • This

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  • page

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  • lists

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  • users

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  • who

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  • are

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  • the

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  • Principal

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  • Investigator(s)

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  • or

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  • the

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  • contact

...

  • person(s)

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  • for

...

  • this

...

  • experiment.

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  • The

...

  • information

...

  • on

...

  • this

...

  • page

...

  • either

...

  • comes

...

  • from

...

  • manual

...

  • data

...

  • entry

...

  • when

...

  • the

...

  • experiment

...

  • was

...

  • created

...

  • or

...

  • comes

...

  • from

...

  • the

...

  • loading

...

  • of

...

  • a

...

  • MAGE-TAB

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  • IDF

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  • file.

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  • Annotation:

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  • This

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  • collection

...

  • of

...

  • pages

...

  • gives

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  • detailed

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  • information

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  • on

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  • the

...

  • experiment

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  • and

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  • biomaterials

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  • on

...

  • which

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  • the

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  • experiment

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  • is

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  • conducted.

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  • It

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  • has

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  • seven

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  • sub

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  • menu

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  • items

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  • (tabs)

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  • that

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  • can

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  • be

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  • categorized

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  • into

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  • three

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  • groups:

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    • Experimental

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    • Design

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    • and

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    • Experimental

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    • Factors:

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    • They

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    • describe

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    • the

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    • experiment

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    • mainly

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    • using

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    • a

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    • controlled

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    • vocabulary,

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    • the

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    • MGED

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    • Ontology

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    • (MO).

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    • Non-MO

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    • terms

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    • can

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    • be

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    • added

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    • as

...

    • well.

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    • The

...

    • information

...

    • on

...

    • these

...

    • two

...

    • pages

...

    • either

...

    • comes

...

    • from

...

    • manual

...

    • data

...

    • entry

...

    • when

...

    • the

...

    • experiment

...

    • was

...

    • created

...

    • or

...

    • comes

...

    • from

...

    • the

...

    • loading

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    • of

...

    • a

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    • MAGE-TAB

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    • IDF

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    • file.

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    • Sources,

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    • Samples,

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    • Extracts,

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    • and

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    • Labeled

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    • Extracts:

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    • They

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    • are

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    • biological

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    • source

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    • materials

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    • (biomaterials)

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    • specified

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    • in

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    • the

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    • MAGE-TAB

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    • format

...

    • (

...

...

    • ).

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    • The

...

    • following

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    • image

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    • (from

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    • the

...

...

...

    • ),

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    • describes

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    • the

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    • relationships

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    • among

...

    • them:

relationships among materialsImage Added
In most cases the biomaterial information comes from the loading of a MAGE-TAB SDRF file. In the SDRF file a user gives proper labels for biomaterials and can also add additional columns describing the characteristics of each biomaterial according to the MAGE-TAB specification. On this set of pages, a user can track the linkage from source materials to labeled extract, then to the final hybridization on microarray chip.

    • Hybridizations: This page captures information related to hybridizations on microarray chips. It establishes linkage between data files collected after hybridization and the corresponding labeled abstracts from which the data are derived. In most cases the hybridization information comes from the loading of a MAGE-TAB SDRF
      Anchor
      _GoBack
      _GoBack
      file.
  • Data: This collection of pages provides access to data loading, processing, and downloading.
    • Data loading and processing: Data are first loaded into the "Manage Data" tab, typically using the MAGE-TAB format. Once they are processed (validated and imported, or classified as supplemental files), they are automatically removed from the "Manage Data" tab and moved to the "Imported Data" tab or the "Supplemental Files" tab.
    • Data download: A user can download the whole dataset or any subset of data here. This page offers three download options:
      • Download Experiment File Packages: it allows download of pre-packaged files.
      • Download Selected Experiment Files: it allows download of a selected set of files.
      • Export Experiment Annotation Packages: it allows the export of experiment annotation in either MAGE-TAB or GEO SOFT (some restrictions apply) format.
  • Publications: This page lists all publications added by the experiment owner. The information on this page either comes from manual data entry or comes from the loading of a MAGE-TAB IDF file.

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