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release=caIntegrator 1.2

entrydate=August 31, 2010

topic=CaIntegrator Usage

Qtext=
*What is the format of caArray supplementary files that can be loaded into caIntegrator? *

Atext=
This knowledge base entry describes the format when creating supplemental files for use by caIntegrator. The supplemental files described here are to be added to an experiment in caArray prior to configuring a study in caIntegrator.

The file itself is a tab-delimited text file. The file extension can be anything, though .txt is typically used. The name of each supplemental file has to be unique within a caArray experiment.

Inside the file, each row in the file contains the data from one reporter. Each column in the file must have a unique header name. (i.e you can not give two different columns the same column name.)

There are 2 supported formats:

  1. SINGLE SAMPLE FORMAT
  2. MULTIPLE SAMPLE FORMAT

SINGLE SAMPLE FORMAT

The format requirements for the single sample format file are following:

  1. Minimum of two required columns.
  2. One column must contain the reporter/probe name.
  3. One column must contain the value be reported by the reporter.
  4. The file can have additional columns, though other than reporter/probe name and value mentioned above, the rest will be ignored.
  5. One SINGLE SAMPLE FORMAT file for each sample in the experiment.

An example of SINGLE SAMPLE FORMAT can be found in the figure below:

MULTIPLE SAMPLE FORMAT

The format requirements for the multiple sample format file are following:

  1. one column must contain the reporter/probe name.
  2. each additional columns are the reporter values such that there is one column per sample.
  3. one MULTIPLE SAMPLE FORMAT file for the whole experiment.
  4. CAVEAT: Currently the MULTIPLE SAMPLE FORMAT is slower to load than the SINGLE SAMPLE FORMAT for platforms other than Agilent Copy Number. This performance issue is expected to be improved in a future release.

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